Abstract:

Abstract:Objective To observe the effects of ACE inhibitor enalaprilat(Ena) and protein kinase C(PKC) inhibitor chelerythrine(Chele)on proliferation,collagen Ⅰ,cell cycle,PKC and cyclinD₁ protein expression of neonatal cardiac fibroblasts(CFb) and to probe its molecular mechanism.Methods CFb of neonatal Wistar rats were divided into control group,AngⅡ group,Chele+AngⅡ group,Chele+AngⅡ+Ena group and AngⅡ+Ena group.CFb were isolated by trypsin digestion method.MTT colorimetric assay was adopted to evaluate cell proliferation,immunocytochemical staining(IC) was used to measure collagen Ⅰ content,Western blotting and flow cytometry were used to detect PKC,cyclinD₁ and cell cycle respectively.Results Compared with AngⅡ group，the MTT value decreased dramatically（P<0.05 or P<0.001）,collagen Ⅰ content decreased（P<0.05 or P<0.01）;the percentage of cell at G₀/G₁ phase increased and the percentage of cells at S phase decreased（P<0.05 or P<0.01）;and the PKC and cyclinD₁ protein expressions were inhibited in Chele,Ena,and Chele+AngⅡ+Ena groups（P<0.05 or P<0.01）.Conclusion The antiproliferation effects of Ena on CFb can be attributed to inhibiting PKC/cyclinD₁ signal pathway possibly.

Key words: enalaprilat;chlerythrine;cyclinD₁;cardiac fibroblast