Abstract:

Abstract:Objective To investigate the relationship between poly(ADP-ribose)glycohydrolase（PARG） and   vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF)  in colorectal carcinoma(CRC) tissues.Methods Immunohistochemical analysis was used to detect poly(ADP-ribose) polymerase(PARP),VEGF and bFGF in 43 cases of human colorectal carcinoma and 10 cases of intestinal mucosa of incisal margin(control group).Western blotting was used to detect the expressions of PARG,PARP,NF-κB,VEGF and bFGF in human Lovo cell line before and after the treatment of PARG inhibitor gallotannin (GLTN).Results The positive rates of PARP,VEGF and bFGF in colorectal carcinoma tissues were 97.67%(42/43),79.07%(34/43),and 81.40%(35/43),respectively;they were significantly higher than those in control group(P<0.05).The expression of PARP had positive correlation with VEGF (r=0.3968,P<0.05) and bFGF (r=0.5610,P<0.05).In GLTN-treated Lovo cell line,the expressions of PARG,PARP,NF-κB,VEGF and bFGF were markedly reduced compared with untreated cells (P<0.01).Conclusion PARG may affect the expression of the factors related to neovascularization in colorectal carcinoma.Its mechanism may be related to the regulation of PARP and affecting the NF-κB activity.

Key words: poly(ADP-ribose) glycohydrolase;poly(ADP-ribose)polymerase;vascular endothelial growth factor;basic fibroblast growth factor;colonic neoplasms