Abstract:

Abstract:Objective To investigate the effect of miRNA-21 antisense oligonucleotide（ASOND） on the proliferation and apoptosis of bladder cancer cell line T24,and provide theoretical basis for treatment of bladder cancer. Methods AS-miRNA-21 mediated by liposome was transfected into T24 cells. As-miRNA-21 group,transfection nonsense series group and control group were set up.The protein expression level of miRNA-21 of the transfected cells was detected  by Western blotting,and the proliferation and apoptosis of the transfected cells were measured by MTT and FCM.Results The miRNA-21 expression level of the tumor cells transfected with  AS-miRNA-21 was decreased,the cell proliferation was inhibited.The proliferation                        
rates of T24 cells in AS-miRNA-21 group,transfection nonsense series group and control group were 53.6%,92.5%,and 100%,respectively; the relative apoptotic rates were 13.8%,1.9% and 2.7%.There were significant differences of the proliferation rate and apoptotic rates between the three groups（P<0.01）.Conclusion Antisense miRNA-21 has the action of proliferation inhibition and apoptosis enhancement on human bladder cancer cell T24. Inhibiting the expression of miRNA-21 may become a prospective gene therapy for bladder cancer.

Key words: bladder neoplasms;miRNA-21;antisense oligonucleotides