Abstract:

Abstract:Objective To evaluate the protective effect of polysaccharides of lentinus edodes (PLE) on immune and anti-oxidative and hematopoietic function of ionizing irradiated mice,and provide theoretical basis for its chinical application.Methods Fifty ICR mice were randomly divided into normal control group(NC),irradiating control group(IC),low dose of PLE irradiating group(800 mg?kg-1),middle dose of PLE irradiating group(1 600 mg?kg-1),and high dose of PLE irradiating group(2 400 mg?kg-1)(n=10).The mice in PLE groups were gaster-poured by PLE for 7 d,the mice in NC group and IC group were gaster-poured by 0.9% sodium chloride solution. The mice in all groups except NC group were irradiated with 2 Gy X-ray on the eighth day.The spleen  index,thymus index,the activity of SOD and content of MDA,the number of WBC,the micronucleus rate of bone marrow polychromalic erythrocytes (PCE)were detected at the 24th hour after radiation.Results Compared with IC group,the  spleen index,thymus index and activity of SOD in high dose of PLE irradiating group were increased markedly(P<0.05); the contents of MDA in  middle and high dose of PLE irradiating groups were increased significantly(P<0.05).The number of WBC in PLE groups was  increased significantly (P<0.05);the micronucleus rates of bone marrow PCE in middle and high dose of PLE irradiating groups were  decreased significantly (P<0.05).Conclusion PLE has an obvious protective effect on damage in  X-ray irradiated mice.

Key words: polysaccharides of lentinus edodes;radioprotection;viscera index;antioxidation function;micronucleus