Abstract:

Abstract:Objective To approach the potential  of differentiation of human placenat derived mesenchymal-like stem cells(HPMSCS )into endothelial cells in vitro.Methods With the agreement of the patients,the HPMSCs were isolated and cultivated from the placenta of the term delivery parturients.The biomorphological changes were observed under inverted microscope.The surface marker was detected by flow cytometry,and through osteoblastic inducing identification and  lypoblastic inducing identification, the multi-directional differentiation potential was defined.The HPMSCs  were treated with  endothelial cell inducing fluid in vitro and induced to differentiate into endothelial cells, and then fluorescent staining was used to dectect the expression of endothelial cell specific  factor Ⅷ (vWF factor).The cell surface marker was detected by flow cytometry after induction.The endothelial cell phenotypes were observed.Results The shape of the cultured HPMSCs changed  from fibroblast-like cells to endothelial-like cells.The expression of the surface marker-vWF was positive  in induced HPMSCs,the expressions of CD31 and CD34  were also positive.Conclusion HPMSCS can be induced to appear the phenotypes of endothelial cells  by this method,which indicates that HPMSCS have the ability to differentiate into endothelial cells in definite conditions in vitro.

Key words: human placenat derived mesenchymal-like stem cells;endothelial cells;induction and differentiation