Abstract:

Abstract:Objective
To study the influence of specific oligodeoxynucleotides (ODN) on the experimental tooth movement in rats and to clarify its role in the reconstruction of periodontal tissues. Methods Fourty male Wistar rats were randomly divided into experiment group (n= 20) and control group (n= 20).The tooth movement models were set up.Then 50μL specific ODN (20 mg•L^-1) was injected into both sides of the buccal gingival mucosa adjacent to the first upper molar in experiment group,the rats in control group were injected by the same amount of PBS in the sam place,once  every three days.4 rats were killed after 3,7,14,21 and 28 d in each group.Then the distance of tooth movement was measured before the models were made and before the rats was killed,and the changes of periodontal tissues were observed by HE staining and the number of osteoclasts was counted. Results The distance of tooth movement in rats were gradually increased. Except for 3 d group,the distances in  experiment group were shorter  than those in  control group（P<0.05 or P<0.01）. HE staining showed that the absorption on pressure side of the first molar alveolar bone and the number of osteoclasts in  experiment  group were less than those in  control group, especially on the days of 3,7 and 14（P<0.05 or P<0.01）.Conclusion The specific ODN can inhibit the tooth movement by reducing the number of OC on the pressure side of alveolar bone,so the specific ODN can influence the reconstruction of periodontal tissues.

Key words: oligonucleotides;orthodontics;tooth movement;periodontal tissue