Abstract:

Objective To study of protective effect of schisandrae chinenesis oil on the islet B cells of type 2 diabetic rats and explore its hypoglycemic effectand mechanism. Methods The high-fat diet combined with the twice injection of l
ow-dose STZ at the ideal interval was used to establish the experimental rat model of type 2 diabetes.The Wistar model rats were randomly divided into diabetic model group and  schisandrae chinenesis oil treatment group,meanwhile control group and normal schisandrae chinenesis oil group were set up. After 6 weeks abdominal injection of rats with schisandrae chinenesis oil,the fasting blood glucose(FBG) and fasting plasma insulin level(FINS) in serum of the rats in various groups were examined;the malondialdehyde(MDA) content,superoxide dismutase(SOD) and catalase(CAT) activities in serum were measured;RT-PCR assay was used to examine the expressions of Bcl-2,Bax and Caspase 3 mRNA. Results Compared with diabetic model group,the level of FBG in schisandrae chinenesis oil gourp was significantly decreased(P<0.05),the FINS level was markedly increased(P<0.05);the serum MDA content,and the SOD and CAT activities were increased(P<0.05)
;the expression of Bcl-2 mRNA was increased(P<0.05),the expression level of Caspase 3 mRNA was decreased(P<0.05),and the Bax mRNA expression had no change. However there were no differences in these indexes between control group and normal schisandrae chinenesis oil group. Conclusion Schisandrae chinenesis oil can significantly reduce blood glucose by decreasing lipid peroxidation,increasing the antioxidant capacity of the d
iabetic rats,regulating apoptosis-related genes,and improving insulin secretion.

Key words: schisandrae chinenesis oil;type 2diabetes;islet B cells;apoptosis;oxidative stress