Abstract:

Objective To observe the expression  characteristics of  cytoketatin19（CK19） and Galectine-3  in thyroid  puncture samples and analyze their applicantion in differential diagnosis for   benign and malignant thyroid disorders,and to explore the feasibility of application of Galectine-3 and CK19 in pathological diagnosis of small puncture samples.Methods The puncture tissues from 21 cases of papillary thyroid carcinoma(PTC) and 16 cases of benign thyroid lesions(BTL) were examined by HE section and the  expressions of Galectin-3,CK19 and Ki67 were detected by EnVision immunohistochemistry. The different expressions of the three proteins were compared between two groups and their roles in the pathological diagnosis in the puncture samples before  thyroid operation were analyzed.Results The positive rates of Galectin-3,CK19 and Ki67 in PTC group was were 90%（18/20）,95%（19/20）,and 60%（12/20），respectively.The positive rates of the three proteins in BTL group were 12.5%（2/16，mildly positve）,31.25%（5/16，mildly positive）, and 62.5%（10/16）,respectively. There were significant differences of Galectin-3 and CK19 between PTC and BTL groups（P<0.01）.There was no significant difference of Ki67 expression between PTC and BTL groups（P>0.05）.Conclusion The expressions of Galectin-3 and CK19 are significantly different between PTC and BTL.Combined detection of these two proteins plays an important role in differential diagnosis of PTC and can improve the diagnosis rate of puncture tissue of thyroid disorders before operation.
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Key words: papillary thyroid carcinoma;Galectin-3;cytoketatin 19;puncture biopsy