Abstract:

Abstract:Objective  To explore the effects of caloric restriction and exercise on lipoprotein particle diameter and distribution of female obesity and provide evidence for obese patients to formulate special therapeutic schedule.
Methods Eighty female obese aged 30-60 years were randomly divided into control group (CG,n=20),diet group (DG,n=20),exercise group (EG,n=20) and diet+exercise group (DEG,n=20).The subjects in DG and EG performed diet and exercise interven
tion respectively and those in DEG simultaneously conducted two intervention style above,while those in CG maintained their routine lifestyle.The test duration was 8 weeks.The lipoprotein particle diameters were measured by polyacrylamide gel
electrophoresis before and after test. Fixation,staining and photograph were made after plasma electrophoresis.Standard curve was determined by taking natural logarithm of standard protein diameter as ordinate and migration lenth as abscissa.
The diameters of low density lipoprotein (LDL) and high density lipoprotein (HDL) particle  were figured out according migration lenth on gel board of the samples.Results Compared with before test,8 weeks after test,the LDL particle diameter was increased (P<0.05),the proportion of small low density lipoprotein (sLDL) particle was decreased (P<0.05) and the proportion of large low density lipoprotein (lLDL) particle was raised (P<0.05),the diameter and distribution of HDL had no significant change (P>0.05) in DG; the HDL particle diameter was elevated (P<0.01),the proportion of small high density lipoprotein (sHDL) particle was reduced (P<0.05) and the proportion of large high density lipoprotein (lHDL) particle was raised (P<0.05),the diameter and distribution of LDL had no significant change (P>0.05) in EG; both of the LDL and HDL particle diameters were  elevated (P<0.01 or P<0.05),the proportions of sLDL and sHDL particles were reduced (P<0.01) and the proportions of lLDL and lHDL particles were  raised (P<0.01) in DEG; all indices in CG showed no significant differences (P>0.05).Conclusion Caloric restriction or exercise could partly have impact on LDL and HDL particle diameters and distribution of female obesity while combination of caloric restriction and exercise may simultaneously improve lipoprotein particle diameter and distribution,thus reduce cardiovascular risk level of female obesity.
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Key words: caloric restriction, exercise, lipoprotein particle, obesity