Abstract:

Abstract:Objective  To explore the effects of cyclosporine A(CsA) and porphyromonas gingivalis-lipopolysaccharide(Pg-LPS) on the area of buccal gingiva of the firstmolar of mandibular and CEJ-ABC distance of rats.Methods Forty rats were equally divided into eight groups.There were 5 rats in each group.The first molar ofmandibular of rats in various groups were injected,from the buccal side,with saline,CsA（2.0 mg/kg）,LPS（10.0,1.0,0.1  μg）,CsA+ LPS（10.0  μg）,CsA+ LPS（1.0  μg）,CsA+ LPS（0.1  μg）,respectively.After 30 d,the mandibles were then  dissected and sectioned for histological and immunocytochemical analysis by haematoxylin andeosin staining,the changes of the distance of CEJ-ABC and the area of buccal gingiva in various groups were observed.Results CsA（2.0 mg/kg） increased the area of thebuccal gum of first molar and the distance of CEJ-ABC，but there was no statistically significant difference compared with control group（P>0.05）.LPS （10.0  μg）alone or combined with CsA  could damage the alveolar bone and increase the distance of CEJ-ABC，and there were  statistically significant differences compared with control group（P<0.05）;but  there was  no significant difference when treated with LPS (1.0,0.1  μg) alone or combined with CsA;and  no obvious change in gingival was found following all of the treatments（P>0.05）.Conclusion  Low dose of CsA（2.0 mg/kg） has no damage on the periodontal tissues of rats,and such a level of CsA may not decrease the periodontal breakdown induced by  high dose  of LPS(10.0 μg).

Key words: cyclosporine A;lipopolysaccharide, rat,sprague-dawley; , gingival, alveolar bone