Abstract:

Objective  To investigate the correlation between 4 single nucleotide polymorphisms（SNPs） of vitamin D receptor (VDR) gene and type 2 diabetes mellitus （T2DM） in Ningxia Han population and to provide theoretical basis for  T2DM prevention.Methods  Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method was carried out to examine the SNPs at rs1544410 (G>A)，rs757343 (G>A)，rs731236 (T>C) and rs739837 (G>T) sites of VDR gene  in 201 T2DM patients (T2DM group) and 210 healthy individuals (control group) in Ningxia Han population.The χ2 test was used to compare the allelic frequencies and genotypic frequencies between two groups.The SHEsis online haplotype analysis software was applied.
Results The genotypic frequencies of rs1544410 and rs757343 had significant differences between T2DM group and control group (P<0.05).The frequency of allele G was higher in T2DM group (93.5%) than that in control group (89.3%), and the difference between  two groups had statistically significant difference (P<0.05).The OR
［95%CI］ value was 1.738 ［1.055-2.865］.There were 16 haplotyes in 4 SNPs in all and  the frequency of haplotype GGCT had significant difference between T2DM group (10.0%) and control group (6.1%)(P<0.05),
with OR ［95%CI］ = 1.723 ［1.03-2.883］.Conclusion The allele G of rs1544410 and the haplotype GGCT of VDR gene may be associated with T2DM susceptibility in Ningxia Han population.
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Key words:  vitamin D receptor gene;diabetes mellitus,type 2; Ningxia Han nationality, single nucleotide polymorphisms