Abstract:

Objective To observe the expressions of apoptosis-related factors in serum and cervix tissue of the patients with different cervical lesions, and to explore their relationships with the clinicopathological characteristics of cervical squamous cell carcinoma. Methods 140 patients with different cervical lesions diagnosed with microsopic examination were selected, including 70 cases of cervical squamous carcinoma, 50 cases of cervical carcinoma in situ (CIN Ⅲ) and 20 cases of cervicitis, and fasting venous blood was also collected and the cervical tissue for microscopic examination was obtained.The protein and mRNA expressions of p53, Fas, TNF-α and cyclin E in serum and different cervical tissues were detected by ELISA, real-time fluorescence quantitative method and immunohistochemical method;the relationships between the expressions of p53, Fas, TNF- α and cyclin E proteins and the clinical stages, pathological types and lymph node metastasis of cervical squamous cell carcinoma were analyzed. Results Compared with the patients with cervicitis, the protein and mRNA expression levels of p53, Fas, and TNF-α in serum and cervix tissue in the patients with cervical squamous cell carcinoma and cervical carcinoma in situ were decreased(P<0.01), but the expression levels of cyclin E mRNA and protein were significantly increased(P<0.01), there was no statistical significance between the patients with cervical squamous cell carcinoma and the patients with cervical carcinoma in situ (P>0.05).The positive expression rates of p53, Fas, TNF- α and cyclin E had positive relations with the clinical stages, pathological types and lymph node metastasis of cervical squamous cell carcinoma(P<0.01). Conclusion The down-regulation of the expression levels of p53, Fas and TNF-α and the up-regulation of cyclin E expression level in the serum and cervix tissue of the patients with cervical squamous cell carcinoma and cervical carcinoma in situ may be associated with the tumor invasion and metastasis of cervical carcinoma, and they may be served as the markers for early diagnosis of cervical cancer;the study provides guidance for the postoperative treatment and prognosis judgement of cervical cancer.

Key words: uterine cervical neoplasms, cervical carcinoma in situ, cervicitis, tumor suppressor gene, apoptosis signal receptor, tumor necrosis factor alpha, cyclin E