Abstract:

Objective To construct the interference vector stably silencing Rhoc gene in the ovarian cancer SKOV3 cells induced by lentivirus,and to observe its inbibitory effect on the expression of RhoC gene in the ovary cancer SKOV3 cells.Methods The ovary cancer SKOV3 cells were divided into psiHIV-RhoC1 group(transfected with Lenti-shRhoC1),psiHIV-RhoC2 group(transfected with Lenti-shRhoC2),negative control group(transfected with Lenti-NC) and blank control group(with no transfection).The expressions of green fluorescence protein in the SKOV3 cells in various groups were detected;the expression levels of RhoC mRNA in the SKOV3 cells in various groups were detected by RT-PCR method.Results There were the expressions of the green fluorescence protein in the SKOV3 cells in psiHIV-RhoC1,psiHIV-RhoC2,and negative control groups. The specific interference vector was constructed and transfected into SKOV3 cells successfully.Compared with bank control group,the expression levels of RhoC mRNA in the SKOV3 cells in psiHIV-RhoC1,psiHIV-RhoC2,and negative control groups were decreased.Conclusion The recombinant lentivirus RhoC shRNA interference vector can suppress the expression of RhoC mRNA in SKOV3 cells effectively.

Key words: RNA interference, lentivirus, ovary neoplasms, RhoC gene