精氨酸-甘氨酸-天冬氨酸多肽表面修饰多孔钽对软骨细胞黏附、增殖和分泌功能的促进作用

doi:10.13481/j.1671-587x.20150315

Abstract

Abstract:

Objective To study the effects of arg-gly-Asp (RGD) peptides coated porous tantalum(Ta) on the adhesion, proliferation and secretion of chondrocytes, and to investigate the feasibility of RGD/chondrocyte/porous tantalum composites for chondral defect repair and regeneration. Methods RGD peptides with different concentrations were loaded to the porous tantalum(Ta) surface by physical absorption and divided into 7 groups:Ta-RGD, Ta-ColⅡ, Ta-RGD/ColⅡ0.2 g·L^-1, Ta-RGD/ColⅡ 1.0 g·L^-1, Ta-RGD/ColⅡ5.0 g·L^-1, Ta-RGD/ColⅡ10.0 g·L^-1 and Pure Ta groups.The articular chondrocytes from immature rabbits were cultured and the 2rd generation chondrocytes were seeded onto porous tantalum.The morphology of RGD/chondrocyte/porous tantalum composites was observed by scanning electron microscopy(SEM).The adhesion rate of chondrocytes was detected by precipitation process, the proliferation level of chondrocytes was measured by MTT assay, and the changes of hydroxyproline level of chondrocytes were checked. Results The adhesion rate of chondrocytes in the porous tantalum modified with RGD peptides was higher than that in the unmodified porous tantalum(P<0.05).The best cell adhesion effect was gained in Ta-RGD/ColⅡ5.0 g·L^-1 group at 4 h and there were significant differences between various groups (P<0.05).The SEM found that the chondrocytes growed best on the surface and inner of porous tantalum and secreted extracellular matrix.The Results of MTT showed that the proliferation level of chondrocytes in the porous tantalum modified with RGD peptides was higher than that in the unmodified porous tantalum(P<0.05) and the best effect was gained in Ta-RGD/ColⅡ1.0 g·L^-1 group at 13 d.The determination of hydroxyproline showed that the hydroxyproline level of chondrocytes in Ta-RGD/ColⅡ1.0 g·L^-1 group was significantly higher than those in other groups(P<0.05). Conclusion The porous tantalum modified with RGD peptides is advantageous to enhance chondrocyte adhesion and proliferation on the surface and inner of porous tantalum and promots the secretion of chondrocytes.

Key words: porous tantalum, arg-gly-asp peptides, adhesion, chondrocytes

CLC Number:

R318.08

GAN Hongquan, WANG Qian, ZHANG Hui, ZHAO Hongkun, WANG Hui, SONG Huiping, BI Cheng, WANG Zhiqiang, LI Qijia. Promotion effect of RGD peptides-grafted porous tantalum on adhesion, proliferation and secretion of chondrocytes in vitro[J].Journal of Jilin University Medicine Edition, 2015, 41(03): 510-516.

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Promotion effect of RGD peptides-grafted porous tantalum on adhesion, proliferation and secretion of chondrocytes in vitro

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