Journal of Jilin University Medicine Edition ›› 2015, Vol. 41 ›› Issue (03): 588-592.doi: 10.13481/j.1671-587x.20150330

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Influence of potential tumor suppressor gene TXNIP in biological characteristics of gastric cancer MKN-45 cells

LI Chunmei1,2, ZHANG Lin2, HOU Yanhong2, LI Nan2   

  1. 1. Department of Postgraduate, Hebei North University, Zhangjiakou 075000, China;
    2. Deparment of Gastroenterology, No.309 Hospital of Chinese PLA, Beijing 100091, China
  • Received:2014-11-14 Published:2015-08-01

Abstract:

Objective To investigate the influence of TXNIP in the biological characteristics of gastric cancer MKN-45 cells, and to clarify the relationships between TXNIP and the carcinogenesis and progression of gastric cancer. Methods The cDNA of TXNIP was subcloned into a constitutive vector pcDNA3.1 followed by transfection in MKN-45 cells by using liposome.RT-PCR and immunocytochemistry were used to detect the expressions of TXNIP gene and protein in MKN-TXNIP cells.Each detection experiment was divided into MKN-TXNIP group, MKN-PC group and MKN-45 group (blank control group).Flow cytometry was used to analyze the apoptosis and cell cycles of gastric carcinoma cells.The cell growth curves and the colony assay were used to detect the growth and proliferation of MKN-45 cells.Transwell chamber was used to detect the invasion. Results Compared with MKN-PC group and MKN-45 group, TXNIP gene could express stably in MKN-TXNIP group.The growth speed of the MKN-45 cells in MKN-TXNIP group was slower than those in MKN-45 group and MKN-PC group(P<0.05).The Results of colony formation assay showed that the colony formation rate in MKN- TXNIP group was lower than those in MKN-45 group and MKN-PC group (P<0.05).The percentage of the cells in G0/G1 phase in MKN-TXNIP group was higher significantly(P<0.05), and the percentage of the cells in G2/M plase was slower than those in MKN-45 group and MKN-PC group.The flow cytometry Results showed that the apoptotic rates of the cells in each group were about 2.0%, and there were no significant differences between various groups(P>0.05).The Results of migration assay suggested that the cell migration rate of the MKN-45 cells in MKN-TXNIP group was significantly lower than those in MKN-45 group and MKN-PC group (P<0.05). Conclusion TXNIP gene can inhibit the growth, proliferation, division and invasion of gastric cancer cells.It can also influence the cell cycle, but it only has little influence in the apoptosis of MKN-45 cells.

Key words: stomach neoplasms, thioredoxin interaction protein, cell cycle, transfection

CLC Number: 

  • R735.2