Notch 1对舌麟状细胞癌Tca8113细胞表皮生长因子受体表达的影响及其意义

doi:10.13481/j.1671-587x.20170410

Abstract

Abstract: Objective: To transfect the tongue squamous cell carcinoma Tca8113 cells with NICD, and to investigate the effects of Notch 1 on Tca8113 cells and the expression of epidermal growth factor receptor (EGFR) in tongue squamous cell carcinoma.Methods: The Notch plasmid pRAMIC-IRES2-EGFP encoding exogenous and control plasmid pIRES2-EGFP were transiently transfected into the Tca8113 cells by Lipofectamine^TM 2000. In the experiment, target plasmid pRAMIC-IRES2-EGFP transfection group, control plasmid pIRES2-EGFP transfection group and non-transfection group were set up. RT-PCR, Western blotting and immunocytochemical method were used to observe the mRNA and protein expression levels of Notch 1 and EGFR. The proliferation activities of Tca8113 cells were detected by MTT assay,and the survival rates were calculated.Results: Compared with non-transfection group and control plasmid pIRES2-EGFP transfection group, the mRNA and protein expression levels of Notch 1 in Tca8113 cells in pRAMIC-IRES2-EGFP group was significantly increased(P<0.05), the mRNA and protein expression levels of EGFR in Tca8113 cells were significantly decreased (P<0.05),and the proliferation rate of Tca8113 cells was significantly decreased(P<0.05) after transfection. There were no significant differences in the expression levels of Notch 1 and EGFR mRNA and protein and the proliferation rates in Tca8113 cells between non-transfection group and pRAMIC-IRES2-EGFP group (P>0.05).Conclusion: The effect of Notch 1 on the proliferation of Tca8113 cells may be related to down-ragulatoin of expression level of EGFR.

Key words: tongue squamous cell carcinoma, Notch 1, epidermal growth factor receptor, transfection

CLC Number:

R739.86

LI Haobo, DU Yong, CHEN Yong, LI Wenjing. Influence of Notch 1 in expression of EGFR in tongue squamous cell carcinoma Tca8113 cells[J].Journal of Jilin University Medicine Edition, 2017, 43(04): 715-719.

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Influence of Notch 1 in expression of EGFR in tongue squamous cell carcinoma Tca8113 cells

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