丝胶对2型糖尿病大鼠肝脏组织中TNF-α和HNF-4α表达的影响

doi:10.13481/j.1671-587x.20150403

Abstract

Abstract:

Objective To investigate the possible mechanisms of sericin in treating diabetes mellitus by observing the effects of sericin on the expressions of tumor necrosis factor-α (TNF-α) and hepatocyte nuclear factor 4 alpha (HNF-4α) in liver tissue of the rats with type 2 diabetes mellitus. Methods 36 male SD rats were randomly divided into normal control group,diabetes model group and sericin treatment group (n=12).The type 2 diabetes mellitus rat models were induced by continuously intraperitoneal injection of streptozotocin(STZ).After successfully establishing the diabetes models,the rats in diabetes model group received any more treatments,and the rats in sericin treatment group were lavaged with sericin (2.4 g·kg^-1·d^-1) for 35 d. SP immunohistochemical staining,Western blotting and RT-PCR were used to detect the TNF-α and HNF-4α expressions in liver tissue of the rats. Results The TNF-α protein immune positive products were tan and brown granules and located in the cytoplasm of liver cells;the HNF-4α immune positive products were tan and located in the nucleus of liver cells. Compared with normal control group,the TNF-α and HNF-4α protein and mRNA expression levels in liver tissue of the rats in diabetes model group were increased obviously (P<0.01).Compared with diabetes model group,the TNF-α and HNF-4α protein and mRNA expression levels in liver tissue of the rats in sericin treatment group were decreased obviously (P<0.01). Conclusion Sericin may protect the liver injury in the rats with diabetes mellitus by down-regulating the TNF-α and HNF-4α expressions in liver tissue.

Key words: sericin, diabetes mellitus,type 2, liver, tumor necrosis factor-α, hepatocyte nuclear factor 4 alpha

CLC Number:

R587.1

LIU Donghui, LIU Meixiao, WANG Xiaojie, ZHANG Yan, YANG Songhe, CHENG Luyang, CHEN Zhihong. Effects of sericin on TNF-α and HNF-4α expressions in liver tissue of rats with type 2 diabetes mellitus[J].Journal of Jilin University Medicine Edition, 2015, 41(04): 685-689.

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Effects of sericin on TNF-α and HNF-4α expressions in liver tissue of rats with type 2 diabetes mellitus

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