胸腺基质淋巴细胞生成素在类风湿关节炎和有关节症状的系统性红斑狼疮患者外周血中的表达及其意义

doi:10.13481/j.1671-587x.20160326

Abstract

Abstract:

Objective: To detect the levels of serum thymic stromal lymphopoietin(TSLP) in the patients with rheumatoid arthritis(RA) or systemic lupus erythemtosus (SLE) with joint symptoms,and to clarify the possible mechanisms of TSLP in the process of RA and SLE. Methods: A total of 40 patients with RA were chosen as RA group,20 SLE patients with joint symptoms were chosen as SLE group,and 20 healthy people were selected as normal control group.The expression levels of serum TSLP of the subjects in various groups were examined by ELISA method and the clinical activity indexes of RA patients were collected,such as C-reaction protein(CRP),erythrocyte sedimentation rate(ESR),cyclic citrullinated peptide(CCP),tender joints count(TJC),swollen joints count(SJC),visual analogue scale(VAS) and disease activity score(DAS28-ESR,DAS28-CRP);the clinical symptoms of SLE patients were collected,such as arthritis,new rash,ulcer and other symptoms; the clinical activity indexes,such as routine blood,24 h urine protein etc.and systemic lupus erythematosus disease activity index(SLEDAI) were estimated.Pearson test was used to analyze the correlation between the serum TSLP level and the indexes of the patients in RA and SLE groups. Results: The level of serum TSLP in the patients with RA was higher than that in normal control group(t=2.73,P<0.05).The level of serum TSLP in the patients with RA was positive correlated with CRP,ESR and anti-CCP antibodies(r=0.504,P=0.001;r=0.550,P<0.0001;r=0.895,P<0.0001); the level of serum TSLP had no correlation with DAS28-ESR and DAS28-CRP(r=0.213,P=0.187;r=0.170,P=0.293).The level of serum TSLP in the patients with positive anti-CCP antibody was higher than that in the patients with negative anti-CCP antibody(F=16.99,P<0.05).The level of serum TSLP in the patients with SLE had no significant difference compared with normal control group(t=0.673,P>0.05),and it had no correlation with SLEDAI(r=0.079,P=0.749). Conclusion: The serum level of TSLP in the patients with RA is obviously increased and TSLP may participate in the pathogenesis of RA and correlate with disease activity.TSLP may be involved in the regulation of anti-CCP antibodies.The changes of serum TSLP level in the RA patients have a certain reference value in monitoring the therapeutic effects.

Key words: thymic stromal lymphopoietin, rheumatoid arthritis, systemic lupus erythemtosus, anti-cyclic citrullinated peptide antibody

CLC Number:

R593.22

GAO Ya, WANG Xiaofei. Expressions of thymic stromal lymphopoietin in serum of patients with rheumatoid arthritis or systemic lupus erythemtosus with joint symptoms and their clinical significances[J].Journal of Jilin University Medicine Edition, 2016, 42(03): 556-560.

References

[1] Zieglar SF,Roan F,Bell BD,et al.The biology of thymic stromal lymphopoietin(TSLP) [J].Adv Pharmacol,2013,66:129-155.

[2] 孙延鹏,卢祖能,王云甫.胸腺基质淋巴细胞生成素与自身免疫性疾病的研究进展[J].医学研究杂志,2013,42(1):183-186.

[3] Moret FM,Hack CE,van der Wurff-Jacobs KMG,et al.Thymic stromal lymphopoietin (TSLP):a novel proinflammatory mediator in rheumatoid arthritis that potently activates CD1c(+) myeloid dendritic cells to attract and stimulate T cells [J].Arthritis Rheum,2014, 66(5):1176-1184.

[4] Usategui A,Criado G,Izquierdo E,et al.A profibrotic role for thymic stromal lymphopoietin in systemic sclerosis [J].Ann Rheum Dis,2013,72(12):2018-2023.

[5] Hillen MR,Kruize AA,Bikker A,et al.Decreased expression of thymic stromal lymphopoietin in salivary glands of patients with primary Sjögren's syndrome is associated with increased disease activity [J].Mod Rheumatol,2016,26(1):105-109.

[6] Koyama K,Ozawa T,Hatsushika K,et al.A possible role for TSLP in inflammatory arthritis [J].Biochem Biophys ResCommun,2007,357(1):99-104.

[7] Hartgring SA,Willis CR,Dean CE Jr,et al.Critical proinflammatory role of thymic stromal lymphopoietin and its receptor in experimental autoimmune arthritis [J].Arthritis Rheum,2011,63(7):1878-1887.

[8] Ellison CA,Lissitsyn YV,Gheorghiu I.Immunomodulatory effects of palifermin (recombinant human keratinocyte growth factor) in an SLE-like model of chronic graft-versus-host disease [J].Scand J Immunol,2012,75(1):69-76.

[9] Friend SL,Hosier S,Nelson A,et al.A thymic stromal cell line supports in vitro development of surface IgM+ B cells and produces a novel growth factor affecting B and T lineage cells[J].Exp Hematol,1994,22(3):321-328.

[10] Soumelis V,Reche PA,Kanzler H,et al.Human epithelial cells trigger dendritic cell mediated allergic inflammation by producing TSLP[J].Nat Immunol,2002,3(7):673-680.

[11] Wei ST, Sun YH,Zong SH,et al.Serum levels of IL-6 and TNF-α may correlate with activity and severity of rheumatoid arthritis[J].Med Sci Monit,2015,21:4030-4038.

[12] Kerkman PF,Fabre E, van der Voort E,et al.Identification and characterisation of citrullinated antigen-specific B cells in peripheral blood of patients with rheumatoid arthritis[J].Ann Rheum Dis,2015.doi:10.1136/annrheumdis-2014-207182.

[13] Tasian SK,Loh ML.Understanding the biology of CRLF2-overexpressing acute lymphoblastic leukemia [J].Crit Rev Oncog,2011,16(1/2):13-24.

[14] Messer L,Alsaleh G,Freyssinet JM,et al.Microparticle-induced release of B-lymphocyte regulators by rheumatoid synoviocytes [J].Arthritis Res Ther,2009,11(2):R40.

[15] 王晓栋,黎莉,沈南,等.Th1/Th2失衡在初发系统性红斑狼疮患者中的研究[J].中华风湿病学杂志,2002,6(5):316-319.

[16] Bengtsson AA,Sturfelt G,Truedsson L, et al.Activation of type Ⅰ inferon system in sustemic lupus erythomatasus correlates with disease activity but not with anuureuval antibodies[J].Lupus,2000,9(9):664-671.

[17] 车国柱,靳雪琴,茹晋丽,等.辅助性T细胞1/辅助性T细胞2失衡在系统性红斑狼疮患者治疗前后变化的研究[J].中国药物与临床,2015,15(8):1071-1073.

[18] Guo S,Wietecha TA,Hudkins KL,et al.Macrophages are essential contributors to kidney injury in murine cryoglobulinemic membranoproliferative glomerulonephritis [J] .Kidney Int,2011,80(9):946-958.

[19] 王晓非,徐京京.系统性红斑狼疮相关骨质疏松[J].中国实用内科杂志,2015,35(10):810-813.

Expressions of thymic stromal lymphopoietin in serum of patients with rheumatoid arthritis or systemic lupus erythemtosus with joint symptoms and their clinical significances

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