黄芩苷对脂多糖诱导的大鼠滑膜RSC-364细胞自噬的抑制作用

doi:10.13481/j.1671-587x.20200311

Abstract

Abstract: Objective: To investigate the effect of baicalin on the autophagy of the synovial RSC-364 cells of the rats induced by lipopolysaccharide (LPS)through PI3k/Akt/mTOR signal pathway, and to clarify its mechanism. Methods: The rat synovial RSC-364 cells in logarithmic growth phase were divided into control group, model group, dexamethasone(DXMS) group, 10μmol·L^-1baicalin group, 20μmol·L^-1 baicalin group and 40μmol·L^-1 baicalin group. The RSC-364 cells in control group were only supplemented with culture medium, and the RSC-364 cells in the other groups were stimulated with 1 mg·L^-1LPS for 12 h to make the inflammatory cell models. The survival rates of RSC-364 cells were detected by MTT assay, and the expression levels of PI3k, Akt, mTOR, Beclin1, and LC3-Ⅱ mRNA in the RSC-364 cells in various groups were detected by RT-PCR method;Western blotting method was used to detect the expression levels of Beclin1, Atg5, Atg7, Atg12, microtubule-associated protein-light chain 3-Ⅱ(LC3-Ⅱ), and P62 proteins in the RSC-364 cells in various groups. Results: Compared with control group, the survival rate of RSC-364 cells in model group was significantly increased (P<0.01), the expression levels of PI3k, Akt, mTOR mRNA and P62 protein in the RSC-364 cells in model group were significantly decreased (P<0.05 or P<0.01), and the expression levels of Atg5, Atg7, Atg12, LC3-Ⅱ, Beclin1 mRNA and proteins were significantly increased (P<0.01); compared with model group, the survival rates of RSC-364 cells in different concentrations of baicalin groups were significantly decreased (P<0.05 or P<0.01), the expression levels of PI3k, Akt, mTOR mRNA and P62 protein in the RSC-364 cells in different concentrations of baicalin groups were significantly increased (P<0.05 or P<0.01), and the expression levels of Atg5, Atg7, Atg12, LC3-Ⅱ and Beclin1 mRNA and proteins were decreased (P<0.05 or P<0.01). Conclusion: Baicalin may inhibit the LPS-induced autophagy of the RSC-364 cells by activating the PI3k/Akt/mTOR signaling pathway.

Key words: baicalin, RSC-364 cells, rheumatoid arthritis synovial fibroblasts, autophagy

CLC Number:

R329.28

WANG Yingying, SUN Lijuan, FAN Ziwei, GU Hong, YOU Xianmei, GUAN Tianhao, ZHANG Chengyi, CHEN Xi. Inhibitory effect of baicalin on autophagy of synovial RSC-364 cells of rats induced by lipopolysaccharide[J].Journal of Jilin University(Medicine Edition), 2020, 46(03): 498-503.

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Inhibitory effect of baicalin on autophagy of synovial RSC-364 cells of rats induced by lipopolysaccharide

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