Journal of Jilin University(Medicine Edition) ›› 2021, Vol. 47 ›› Issue (2): 460-468.doi: 10.13481/j.1671-587X.20210227
• Research in clinical medicine • Previous Articles Next Articles
Jie WU1,Xuehua YANG2,Ling MA1,Wenqiang FAN1,Dongdong FU1,Xiao GAO1,Shufei ZUO1,Shu LIANG1,Yilu QIN1,Peishan WANG3(),Jinyan GUO4
Received:
2020-05-22
Online:
2021-03-28
Published:
2021-03-25
Contact:
Peishan WANG
E-mail:ccff-218000@163.com
CLC Number:
Jie WU,Xuehua YANG,Ling MA,Wenqiang FAN,Dongdong FU,Xiao GAO,Shufei ZUO,Shu LIANG,Yilu QIN,Peishan WANG,Jinyan GUO. Effect of miR-26a-5p on apoptosis of human rheumatoid arthritis fibroblast-like synovial cells through JAK2/STAT3 signaling pathway[J].Journal of Jilin University(Medicine Edition), 2021, 47(2): 460-468.
Tab.1
Expression levels of miR-26a-5p mRNA in RA-FLSs and levels of IL-1β, IL-6 and TNF-α in culture fluid in various groups"
Group | miR-26a-5p | IL-1β[ρB/(ng·L-1)] | IL-6[ρB/ (ng·L-1)] | TNF-α[ρB/ (ng·L-1)] |
---|---|---|---|---|
Blank control | 1.00±0.00 | 15.53±1.64 | 92.21±4.17 | 274.56±26.39 |
NC-mimic | 0.99±0.01 | 15.36±1.39 | 92.18±3.97 | 273.12±27.08 |
miR-26a-5p mimic | 3.84±0.24*△ | 11.87±1.05*△ | 72.56±3.45*△ | 228.25±19.23*△ |
NC-inhibitor | 1.01±0.02 | 15.45±1.73 | 91.87±4.52 | 273.87±24.62 |
miR-26a-5p inhibitor | 0.34±0.00*# | 19.16±2.01*# | 122.49±7.23*# | 298.56±29.32*# |
F | 92.097 | 132.724 | 87.193 | 72.372 |
P | <0.01 | <0.01 | <0.01 | <0.01 |
Tab.2
Proliferation activities of RA-FLSs in various groups"
Group | Proliferation activity | ||||
---|---|---|---|---|---|
(t/h) 0 | 12 | 24 | 48 | 72 | |
Blank control | 0.24±0.01 | 0.30±0.02 | 0.52±0.04 | 0.64±0.05 | 0.81±0.05 |
NC-mimic | 0.23±0.02 | 0.30±0.01 | 0.51±0.03 | 0.63±0.04 | 0.80±0.07 |
miR-26a-5p mimic | 0.23±0.00 | 0.29±0.01 | 0.31±0.04*△ | 0.38±0.02*△ | 0.42±0.03*△ |
NC-inhibitor | 0.24±0.02 | 0.29±0.00 | 0.52±0.03 | 0.64±0.05 | 0.80±0.06 |
miR-26a-5p inhibitor | 0.23±0.01 | 0.30±0.02 | 0.69±0.04*# | 0.81±0.06*# | 0.98±0.10*# |
F | 0.203 | 0.117 | 34.472 | 50.452 | 84.324 |
P | 0.912 | 0.936 | <0.01 | <0.01 | <0.01 |
Tab.4
Expression levels of Bcl-2, Bax, JAK2, p-JAK2,STAT3 and p-STAT3 proteins in RA-FLSs in various groups (n=3,x±s)"
Group | Bcl-2 | Bax | JAK2 | p-JAK2 | STAT3 | p-STAT3 |
---|---|---|---|---|---|---|
Blank control | 0.76±0.05 | 0.37±0.01 | 0.51±0.03 | 1.15±0.04 | 0.79±0.06 | 0.45±0.03 |
NC-mimic | 0.77±0.06 | 0.38±0.02 | 0.52±0.05 | 1.19±0.07 | 0.80±0.05 | 0.46±0.03 |
miR-26a-5p mimic | 0.14±0.00*△ | 0.72±0.05*△ | 0.21±0.00*△ | 0.79±0.05*△ | 0.20±0.01*△ | 0.17±0.00*△ |
NC-inhibitor | 0.77±0.04 | 0.37±0.02 | 0.52±0.04 | 1.20±0.11 | 0.79±0.02 | 0.45±0.02 |
miR-26a-5p inhibitor | 2.01±0.13*# | 0.13±0.01*# | 1.36±0.08*# | 2.24±0.17*# | 1.56±0.04*# | 0.70±0.05*# |
F | 81.281 | 68.027 | 120.338 | 79.092 | 87.877 | 56.302 |
P | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 |
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