Abstract:

Objective: To study the inhibitory effect of deoxyschizandrin on the growth of brain glioma C6 cells,and to explore its mechanism. Methods: The rat glioma C6 cells were cultured and divided into control group,50,100,and 200 mg·L^-1 deoxyschizandrin groups. The proliferation rates of C6 cells were examined by MTT assay;the changes of cell cycles were examined by flow cytometry;the expression levels of CyclinD1,Bax,Bcl-2 and Caspase-3 proteins in supernant were detected by ELISA assay. Results: Compared with control group,the proliferation rates at 24 and 48 h in 50,100, and 200 mg·L^-1 deoxyschizandrin groups were significantly decreased(P < 0.01),and the proliferation rates at 72 h in 100 and 200 mg·L^-1 deoxyschizandrin groups were significantly decreased(P < 0.05 or P < 0.01).Compared with control group,the percentage of cells at SubG₁ phase in 200 mg·L^-1 deoxyschizandrin group was increased (P < 0.05),and the percentage of cells at S phase was decreased (P < 0.05). Compared with control group,the expression levels of CyclinD1 in 100 and 200 mg·L^-1deoxyschizandrin groups were decreased (P < 0.01 );the expression levels of Bax protein in deoxyschizandrin groups were increased(P < 0.05 or P < 0.01),and the expression level of Bcl-2 protein in 200 mg·L^-1 deoxyschizandrin group was decreased(P < 0.01),and the Bax/Bcl-2 value in deoxyschizandrin groups were increased(P < 0.01 );the expression level of Caspase-3 protein in 200 mg·L^-1 deoxyschizandrin group was increased(P < 0.01). Conclusion: Deoxyschizandrin could inhibit the growth of glioma cells through down-regulating the expression levels of CyclinD1 protein and up-regulating the expression levels apoptotic factors Bax and Bcl-2.

Key words: deoxyschizandrin, glioma cells, CyclinD1, Bax/Bcl-2