Th17转录因子RORγt和BATF及Th17相关细胞因子在中性粒细胞亚型哮喘发病中的作用

doi:10.13481/j.1671-587x.20170121

Abstract

Abstract:

Objective: To detect the expression levels of retinoic acid-related orphan receptor γt (RORγt),B cell activating transcription factor(BATF) in sputum of the patients with neutrophilic asthma(NA),Th17 cells-related cytokines such as interleukin-8(IL-8),IL-17, and IL-22 in peripheral blood,and to investigate their effects in the pathogenesis of NA.Methods: Fifty-six patients with asthma were selected as subjects and the sputum was induced with 45% normal saline.According to the results of sputum smear by MGG staining,they were divided into eosinophilic asthma group (EA,n=26) and NA group (n=30).28 cases of healthy volunteers were selected as control group. The expression levels of IL-8,IL-17, and IL-22 in serum were detected by ELISA and the expressions of BATF and RORγt mRNA in sputum were determined by RT-PCR. And T-lymphocytes were collected from the peripheral blood mononuclear cells (PBMC),and then the percentages of Th17 cells were determined by flow cytometry.Results: Compared with NC and EA groups,the levels of IL-8,IL-17, and IL-22 in serum of the patients in NA group were significantly increased (P<0.05),but there were no significant differences between EA and NC groups(P>0.05).Compared with NC and EA groups,the expression levels of BATF and RORγt mRNA were significantly increased (P<0.01); compared with NC and EA groups, the percentage of Th17 cells in PBMC of the pathents in NA group was significantly increased (P<0.01),but there was no significant difference between EA and NC groups(P>0.05).Conclusion: RORγt and BATF involve in the onset and development of airway inflammation in the patients with NA,and Th17 cells as well as Th17-related cytokines such as IL-17 and IL-22 may be closely associated with systematic resposes of NA.

Key words: neutrophilic asthma, T helper cells 17, retinoic acid-related orphan receptor γt, B cell activating transcription factor

CLC Number:

R562.25

WANG Guoqiang, WANG Yubo, ZHANG Weijie, WANG Xinran, ZHANG Wenjin, WU Tian, GUAN Xuewa, CHEN Fang, ZHENG Jingtong, WANG Fang. Effects of Th17 transcription factors RORγt, BATF and Th17-related cytokines in pathogenesis of patients with neutrophilic asthma[J].Journal of Jilin University Medicine Edition, 2017, 43(01): 106-110.

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Effects of Th17 transcription factors RORγt, BATF and Th17-related cytokines in pathogenesis of patients with neutrophilic asthma

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