沉默线粒体核糖体蛋白L35基因对人食管癌TE-1细胞生长的影响

doi:10.13481/j.1671-587x.20190106

Abstract

Abstract: Objective: To investigate the effect of lentivirus-mediated silencing of mitochondrial ribosomal protein L35 (MRPL35) gene on the growth of human esophageal cancer TE-1 cells,and to clarify its mechanism.Methods: Three kinds of human esophageal cancer cells, TE-1, ECA109 and KYSE150, were selected. The relative expression levels of MRPL35 mRNA in three kinds of cells by real-time quantitative PCR. The esophageal cancer TE-1 cells were divided into shMRPL35 group and shCtrl group,and the cells were infected with si-RNA lentivirus and si-RNA lentivirus; the esophageal cancer cell line stably silenting the MRPL35 gene was established. Real-time quantitative PCR and Western blotting methods were used to detect the efficiency of MRPL35 gene silencing.The cell growth curves in various groups were detected by CCK-8 method, and the apoptotic rates were detected by flow cytometry after Annexin Ⅴ-PE/7AAD double staining.Results: Three kinds of esophageal cancer cells expressed MRPL35 gene, and the expression levels were not statistically significant between them(P>0.05). The results of real-time quantitative PCR and Western blotting methods showed that the mRNA and protein levels of MRPL35 in the TE-1 cells in shMRPL35 group were significantly lower than those in shCtrl group(P<0.05). Compared with shCtrl group, the cell growth speed in shMRPL35 group was decreased (P<0.05), and the apoptotic rate was significantly increased (P<0.01).Conclusion: Silencing MRPL35 gene can inhibit the proliferation of esophageal cancer TE-1cells and plays a role through the apoptotic pathway.

Key words: mitochondrial ribosomal protein L35, esophageal cancer TE-1 cells, apoptosis

CLC Number:

R735.1

WANG Aifu, ZHANG Qi, ZHANG Daoming, XIU Yuting, DING Yaming, LIU Linlin. Effect of silencing mitochondrial ribosomal protein L35 gene on growth of human esophageal cancer TE-1 cells[J].Journal of Jilin University(Medicine Edition), 2019, 45(01): 28-32.

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Effect of silencing mitochondrial ribosomal protein L35 gene on growth of human esophageal cancer TE-1 cells

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