蚓激酶对胃癌SGC7901细胞增殖抑制和凋亡诱导作用

doi:10.13481/j.1671-587x.20190102

Abstract

Abstract: Objective: To investigate the regulatory effect of lumbrukinase (LBK) on the gastric cancer SGC7901 cells, and to clarify its mechanism.Methods: The SGC7901 cells in the logarithmic growth phase were selected and divided into control group and 2,4,8 U·mL^-1 LBK groups. MTT assay was used to detect the inhibitory rates of proliferation of SGC7901 cells in various groups in vitro at different time (24, 48 and 72 h). Cell scratch assay was used to detect the migration abilities of the SGC7901 cells in vitro in various groups.Flow cytometry was used to determine the apoptotic rates of SGC7901 cells and the pencentages of cells at different cell cycles in various groups. The expression levels of Bcl-2, Bax, and caspase-3 in the SGC7901 cells in various groups were detected by Western blotting method.Results: The results of MTT assay showed that compared with control group, the inhibitory rates of SGC7901 cells in different doses of LBK groups after treated for 24,48 and 72 h were increased(P<0.01). The cell scratch assay results showed that compared with control group, the migration distances of SGC7901 cells in 4 and 8 U·mL^-1 LBK groups were increased significantly(P<0.01).The flow cytometry results showed that compared with control group,the apoptotic rates of SGC7901 cells in 4 and 8 U·mL^-1 LBK groups were increased significantly(P<0.01);the percentages of cells in G₁ and S phases were decreased(P<0.01);the percentages of cells in G₂ phase were increased(P<0.01).The results of Western blotting method showed that compared with control group, the Bcl-2 protein expression level in the SGC7901 cells in 8 U·mL^-1 LBK group was decreased (P<0.05); the Bax and caspase-3 protein expression levels were increased (P<0.05).Conclusion: LBK can inhibit the proliferation and migration abilities of SGC7901 cells in vitro and induce the apoptosis; its mechanism is achieved through the regulation of expression levels of Bcl-2, Bax, and caspase-3 proteins.

Key words: lumbrokinase, SGC7901 cells, cell scratch, apoptosis, cell cycle

CLC Number:

R735.2

LIU Dan, YANG Xiaobo, WANG Ying, YU Lihong, SHI Yan, ZHANG Yingjie, KANG Wanjun. Inhibitory effect of lumbrokinase on proliferation and induction on apoptosis of gastric cancer SGC7901 cells[J].Journal of Jilin University(Medicine Edition), 2019, 45(01): 7-11.

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Inhibitory effect of lumbrokinase on proliferation and induction on apoptosis of gastric cancer SGC7901 cells

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