LincRNA-p21敲减对胃癌细胞生长和转移的影响及其作用机制

doi:10.13481/j.1671-587x.20200210

Abstract

Abstract: Objective: To investigate the effects of LincRNA-p21 knockdown on the proliferation, migration and invasion of gastric cancer cells, and to elucidate their mechanisms. Methods: The expression levels of LincRNA-p21 mRNA in the gastric cancer tissue, adjacent tissue, three types of gastric cancer cells (MGC-803, MKN-45 and SGC-790 cells) and normal gastric mucosal epithelial cells GES-1 were detected by real-time quantitative polymerase chain reaction (RT-qPCR). The MGC-803 cells were used as the subjects and were divided into sh-NC group, sh-LincRNA-p21 group and AG490+sh-LincRNA-p21 group. The MGC-803 cells in sh-NC group were infected with sh-NC by lentivirus; the MGC-803 cells in sh-LincRNA-p21 group were infected with sh-LincRNA-p21 by lentivirus; the MGC-803 cells in AG490+sh-LincRNA-p21 group were infected with sh-LincRNA-p21 by lentivirus, and then treated with 10 μg·L^-1 AG490. The percentages of 5-ethynyl-2'-deoxyuridine (EdU) incorporation of the MGC-803 cells in various groups were detected by EdU incorporation assay. The viabilities of MGC-803 cells in various groups were measured by CCK-8 assay. The number of invasion and migration of MGC-803 cells in various groups was detected by Transwell assay. The expression levels of p-JAK1, p-STAT3 and p-STAT5 proeteins in the MGC-803 cells in sh-NC group and sh-LincRNA-p21 group were detected by Western blotting method. The MGC-803 cells from sh-NC group and sh-LincRNA-p21 group were subcutaneouly transplanted into the neck of the BALB/c nude mice, then the volumes and weights of the tumors of the mice were measured. Results: Compared with adjacent tissue, the expression level of LincRNA-p21 mRNA in gastric cancer tissue was significantly decreased (P<0.01); compared with the GES-1 cells, the expression levels of LincRNA-p21 mRNA in the MGC-803, MKN-45 and SGC-790 cells were significantly decreased (P<0.01).Compared with sh-NC group, the percentage of EdU incorporation, the cell viability, the number of migration and invasion cells, the expression levels of p-JAK1, p-STAT3 and p-STAT5 proteins in the MGC-803 cells in sh-LincRNA-p21 group were significantly increased (P<0.05 or P<0.01).Compared with sh-LincRNA-p21 group, the cell viability and the number of migration and invasion of the MGC-803 cells in AG490+sh-LincRNA-p21 group were significantly decreased (P<0.05 or P<0.01).The results of the tumorigenesis of nude mice showed that compared with sh-NC group, the volume and weight of the tumor of the mice in sh-LincRNA-p21 group were significantly increased (P<0.05 or P<0.01). Conclusion: LincRNA-p21 knockdown can significantly promote the growth and metastasis of gastric cancer cells, and this promotion may be related to promoting the activity of JAK-STAT signaling pathway.

Key words: LincRNA-p21, stomach neoplasms, JAK-STAT signaling pathway, cell proliferation, cell migration, cell invasion

CLC Number:

R735.2

XU Jianguo, CAO Hongtao, ZHANG Zilong, JI Baoyan, WANG Chunqiu, LI Shengdong, LIU Guoqing. Effects of LincRNA-p21 knockdown on growth and metastasis of gastric cancer cells and their mechanisms[J].Journal of Jilin University(Medicine Edition), 2020, 46(02): 266-273.

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Effects of LincRNA-p21 knockdown on growth and metastasis of gastric cancer cells and their mechanisms

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