Journal of Jilin University(Medicine Edition) ›› 2020, Vol. 46 ›› Issue (6): 1177-1181.doi: 10.13481/j.1671-587x.20200611

• Research in basic medicine • Previous Articles     Next Articles

Regulatory effect of gardenoside on sleep disorder in rats with Parkinson’s disease and its mechanism

Xiaofeng LUO1,Yao LI2,Jiang HU3,Chenhao ZHANG2()   

  1. 1.Department of Physiology,College of Basic Medical Sciences,Jilin Medical University,Jilin 132013,China
    2.Department of Pathogeny Technology,College of Medical Laboratory,Jilin Medical College,Jilin 132013,China
    3.Department of Stomatology,Affiliated Hospital,Jilin Medical College,Jilin 132013,China
  • Received:2020-04-30 Online:2020-11-28 Published:2021-01-27
  • Contact: Chenhao ZHANG E-mail:zhangchenhao0618@163.com

Abstract: Objective

To discuss the effect of gardenoside on the sleep disorder in the Parkinson’s disease (PD) rats,and to explore its possible mechanism.

Methods

Seventy Wistar rats were selected and 6-hydroxydopa(6-OHDA) was used to establish the PD rat models. After 4 weeks, the PD models were identified by rotation test induced by intraperitoneal injection of apomorphine.After the models were successfully constructed, the PD model rats were divided into model group, low dose of gardenoside group and high dose of gardenoside group, and there were 10 rats in each group;another 10 normal rats were used as normal control group.The rats in low and high doses of geniposide groups were given geniposide solution (50 and 100 mg ? kg-1) by gavage every day. The rats in normal control group and model group were given the same amount of normal saline for 4 weeks.The sleep time of the rats in various groups was recorded using embedded electroencephalogram(EEG)and electromyography(EMG) electrodes,and the levels of dopamine in striatum of the rats in various groups were analyzed by high performance liquid chromatography (HPLC).

Results

Compared with normal control group, the total sleep time, slow wave sleep time and fast wave sleep time of the rats in model group were significantly shortened (P<0.01), and the awaken time was significantly prolonged (P<0.05).Compared with model group, the total sleep time,slow wave sleep time and fast wave sleep time of the rats in low and high doses of gardenoside groups were significantly prolonged (P<0.05), while the awaken time was significantly shortened (P<0.05). Compared with model group, the levels of dopamine in striatum of the rats in low and high doses of gardenoside groups were significantly increased (P<0.05).

Conclusion

Gardenoside can improve the sleep distorder in the PD model rats, and its mechanism may be related to increasing the level of dopamine in striatum.

Key words: Parkinson’s disease, sleep disorders, gardenoside, slow wave sleep, fast wave sleep

CLC Number: 

  • R392.12