Journal of Jilin University(Medicine Edition) ›› 2021, Vol. 47 ›› Issue (2): 344-351.doi: 10.13481/j.1671-587X.20210213
• Research in basic medicine • Previous Articles Next Articles
Haoxuan TANG1,2,Nanfeng MENG1,2,Meiling DU1,2,Wei WANG1,2,Tao HE1,2()
Received:
2020-07-23
Online:
2021-03-28
Published:
2021-03-25
Contact:
Tao HE
E-mail:manfred_ht@163.com
CLC Number:
Haoxuan TANG,Nanfeng MENG,Meiling DU,Wei WANG,Tao HE. Inhibitory effect of new VEGF monoclonal antibody combined with all-trans retinoic acid on proliferation of breast cancer MCF-7 cells[J].Journal of Jilin University(Medicine Edition), 2021, 47(2): 344-351.
Tab.2
Cytotoxicities of different concentrations of combined drugs to MCF-7 cells detected by MTT method"
Group | Cytotoxicity | |
---|---|---|
Control | 0.30±0.013 | |
1 μmol·L-1 ATRA+15 mg·L-1 VEGF-antibody | 0.32±0.011 | |
5 μmol·L-1 ATRA+15 mg·L-1 VEGF-antibody | 0.32±0.005 | |
1 μmol·L-1 ATRA+20 mg·L-1 VEGF-antibody | 0.34±0.011* | |
5 μmol·L-1 ATRA+20 mg·L-1 VEGF-antibody | 0.36±0.014* | |
10 μmol·L-1 ATRA+10 mg·L-1 VEGF-antibody | 0.36±0.020* | |
10 μmol·L-1 ATRA+15 mg·L-1 VEGF-antibody | 0.38±0.020** | |
15 μmol·L-1 ATRA+10 mg·L-1 VEGF-antibody | 0.43±0.018** | |
10 μmol·L-1 ATRA+20 mg·L-1 VEGF-antibody | 0.51±0.019** |
Tab.5
Expression levels of Bcl-2,Bax, and NF-κB proteins in MCF-7 cells in various groups"
Group | Bcl-2 protein | Bax protein | NF-κB protein |
---|---|---|---|
Control | 0.139 4±0.007 6 | 0.191 9±0.016 4 | 0.174 9±0.017 9 |
VEGF-antibody | 0.122 2±0.007 6** | 0.379 4±0.052 6** | 0.172 9±0.014 5 |
ATRA | 0.096 5±0.005 8** | 0.646 0±0.065 5**△△ | 0.117 6±0.004 9*△△ |
ATRA+VEGF-antibody | 0.079 0±0.006 0**△△# | 0.844 2±0.072 7**△△ | 0.129 8±0.005 9*△ |
P | <0.01 | <0.01 | <0.05 |
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