C19ORF12对胃癌MKN45细胞增殖和化疗药敏感性的影响及其机制

doi:10.13481/j.1671-587X.20210319

Abstract

Abstract: Objective

To discuss the effect of C19ORF12 on the proliferation and chemo-sensitivity of the gastric cancer MKN45 cells，and to clarify its mechanism.

Methods

The gastric cancer MKN45 cells were divided into control group and C19ORF12 group. The proliferation activities of the gastric cancer MKN45 cells in two groups were detected by CCK-8 method； the number of colony formation of the gastric cancer MKN45 cells in two groups was detected by colony formation assay；the survival rates of the gastric cancer MKN45 cells after treated with chemotherapeutic drugs doxorubicin or hydrogen peroxide（H₂O₂） in two groups were detected by CCK-8 method； the apoptotic rates of the gastric cancer MKN45 cells in two groups were detected by flow cytometry； the subcellular localization of C19ORF12 in the gastric cancer MKN45 cells was detected by laser confocal microscope.The expression difference of C19ORF12 gene in Cancer Genome Atlas （TCGA） between normal gastric and gastric cancer tissues was analyzed by GEPIA.The effect of different expression level of C19ORF12 on the prognosis of gastric cancer patients was retrospectively analyzed by KM-plot.

Results

Compared with control group， the proliferation activity and the number of colone formation of the gastric cancer MKN45 cells in C19ORF12 group were significantly increased （P<0.01）. Compared with control group， the survival rate of gastric cancer MKN45 cells in C19ORF12 group after treated with doxorubicin or H₂O₂ were increased（P<0.05 or P<0.01），and the apoptotic rates were decreased（P<0.01）. The C19ORF12 was mainly expressed in the mitochondrion.The bioinformatics analysis results showed that compared with normal gastric tissue， the level of C19ORF12 mRNA in gastric cancer tissue was increased（P<0.01）. Compared with the patients with low expression of C19ORF12， the overall survival time of gastric cancer patients with high expression of C19ORF12 was significantly decreased（P<0.01）.

Conclusion

C19ORF12 can promote the proliferation of gastric cancer cells and reduce the sensitivity of gastric cancer cells to chemotherapeutic drugs. C19ORF12 can be used as a potential target for tumor therapy.

Key words: chromosome 19 open reading frame 12, gastric neoplasm, cell proliferation, doxorubicin, chemo-sensitivity, reactive oxygen species

CLC Number:

R735.2

Ying YANG, Wei ZHAO, Dan LYU. Effect of C19ORF12 on proliferation and chemo-sensitivity of gastric cancer MKN45 cells and its mechanism[J].Journal of Jilin University(Medicine Edition), 2021, 47(3): 687-693.

Figures/Tables 8

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Effect of C19ORF12 on proliferation and chemo-sensitivity of gastric cancer MKN45 cells and its mechanism

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