靶向沉默热休克蛋白27对口腔鳞状细胞癌CAL27细胞侵袭和迁移的作用及其机制

doi:10.13481/j.1671-587X.20210421

Abstract

Abstract: Objective

To investigate the effects of heat shock protein 27 （HSP27） on the invasion and migration of oral squamous cell carcinoma （OSCC） CAL27 cells， and to clarify their mechanisms.

Methods

The CAL27 cells were divided into control group ［transfected with negative control interference RNA（NC-siRNA）］and experiment group［transfected with HSP27 small interference RNA（HSP-siRNA）］.Real-time fluorescence quantitative PCR （RT-qPCR） and Western blotting methods were used to detect the siRNA interference efficiency in the CAL27 cells. Cell scratch assay was used to detect the scratch healing rates of CAL27 cells. Transwell chamber experiment was used to detect the number of invasion and migration CAL27 cells in two groups. RT-qPCR and Western blotting methods were used to detect the mRNA and protein expression levels of epithelial mesenchymal transformation （EMT） marker N-cadherin in the CAL27 cells in two groups.

Results

Compared with control group， the expression levels of HSP27 mRNA and protein in the CLA27 cells in experiment group were significantly decreased （P<0.01）.The results of cell scratch experiment showed that compared with control group， the scratch healing rate of the CAL27 cells in experiment group was significantly decreased （P<0.01）. The results of Transwell chamber experiment showed that compared with control group， the number of invasion and migration cells in experiment group was significantly reduced （P<0.01）. Compared with control group， the expression levels of N-cadherin mRNA and protein in the CAL27 cells in expermient group were significantly reduced （P<0.05）.

Conclusion

Targeted silencing of HSP27 can lead to the reduced migration and invasion abilities of the OSCC CAL27 cells， which may be related to the inhibition of EMT.

Key words: heat shock protein 27, oral squamous cell carcinoma, cell invasion, cell migration, epithelial-mesenchymal transition

CLC Number:

R739.8

Juan CAO,Weibo LI,Xiu GUO,Bo LI,Chunling DONG. Effects of targeted silencing of heat shock protein 27 on invasion and migration of oral squamous cell carcinoma CAL27 cells and their mechanisms[J].Journal of Jilin University(Medicine Edition), 2021, 47(4): 971-977.

Figures/Tables 8

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Effects of targeted silencing of heat shock protein 27 on invasion and migration of oral squamous cell carcinoma CAL27 cells and their mechanisms

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