头孢曲松对蛛网膜下腔出血大鼠星形胶质细胞活化和炎症反应的影响

doi:10.13481/j.1671-587X.20210616

Abstract

Abstract: Objective

To investigate the neuroprotective effect of ceftriaxone （CEF） in the rats with subarachnoid hemorrhage （SAH），and to clarify its possible mechanism.

Methods

A total of 48 male SD rats were randomly divided into sham operation group， SAH group， and CEF group， and there were 16 rats in each group. The SAH rat model was established by improved endovascular puncture. The rats in CEF group were injected intraperitoneally 5 d before the establishment of SAH model， and the drug dose of CEF was 200 mg·kg^－1.After the establishment of SAH model for 24 h， the neurological function scores of the rats in various groups were evaluated. The morphology of nerve cells in brain tissue of the rats in various groups was observed by HE staining， and the numbers of necrosis nerve cells were calculated. The numbers of activated astrocytes in brain tissue of the rats in various groups were examined by immunohistochemistry. The levels of interleukin-1β（IL-1β）， interleukin-33（IL-33）， interleukin-6（IL-6）， and tumor necrosis factor-α（TNF-α） in brain tissue of the rats in various groups were detected by ELISA method.

Results

Compared with sham operation group， the neurological function score of the rats in SAH group was significantly decreased （P<0.05）. Compared with SAH group， the neurological function score of the rats in CEF group was significantly increased （P<0.05）. The HE staining results showed that the distribution of nerve cells in brain tissue of the rats in sham operation group was orderly and regular，the cells were symmetrical， and the nucleus was in the middle and stained clearly； the nerve cells in brain tissue of the rats in SAH group were disordered， irregular and cone-shaped， and the nuclear were shrinkaged， ruptured and dissolved； in CEF group， the structure of nerve cells in brain tissue was intact and the nuclear pyknosis was alleviated. Compared with sham operation group， the number of necrosis nerve cells in brain tissue of the rats in SAH group was significantly increased （P<0.05）；compared with SAH group， the number of necrosis nerve cells in brain tissue of the rats in CEF group was significantly decreased （P<0.05）. The immunohistochemical staining results showed that most of the activated astrocytes were “branched” in shape， with small cell bodies， mainly cytoplasmic staining， and the processes were thickened and lengthened with different lengths. Compared with sham operation group， the number of activated astrocytes in brain tissue of the rats in SAH group was significantly increased （P<0.05）； compared with SAH group， the number of activated astrocytes in brain tissue of the rats in CEF group was significantly decreased （P<0.05）.The ELISA assay results showed that the levels of IL-1β， IL-33， IL-6 and TNF-α in brain tissue of the rats in SAH group were significantly higher than those in sham operation group （P<0.05）；compared with SAH group， the levels of IL-1β， IL-33， IL-6，and TNF-α in brain tissue of the rats in CEF group were significantly decreased （P<0.05）.

Conclusion

CEF can reduce the necrosis of nerve cells in brain tissue of the rats with SAH， improve the neurological function score of the rats， and has a neuroprotective effect； its mechanism may be related to inhibiting the activation of astrocytes and alleviating the inflammatory reaction.

Key words: subarachnoid hemorrhage, ceftriaxone, inflammatory reaction, astrocytes

CLC Number:

R743.35

Zehua GONG,Junjie LIU,Jiwei XU,Jianmin LI. Effects of ceftriaxone on activation of astrocytes and inflammation reaction in rats with subarachnoid hemorrhage[J].Journal of Jilin University(Medicine Edition), 2021, 47(6): 1462-1468.

Figures/Tables 6

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Tab.2

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Tab.3

Fig. 3

References 22

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Group	Garcia score
Sham operation	17.60±0.52
SAH	7.00±0.82^*
CEF	10.70±0.95^△

Group	Number of necrosis nerve cells
Sham operation	1.59±0.34
SAH	45.85±3.81^*
CEF	31.34±3.56^△

Group	Number of activated astrocytes
Sham operation	5.35±1.03
SAH	17.22±2.69^*
CEF	11.95±1.42^△

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Effects of ceftriaxone on activation of astrocytes and inflammation reaction in rats with subarachnoid hemorrhage

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