白藜芦醇对喉癌Hep-2细胞顺铂敏感性的增强作用及其机制

doi:10.13481/j.1671-587x.20150214

Abstract

Abstract:

Objective To investigate the enhancement effect of resveratrol on the sensitivity of laryngeal carcinoma Hep-2 cells to cisplatin(DDP) and its mechanism.Methods The Hep-2 cells were randomly divided into blank control group and different doses(3,6,12, and 24 μmol·L^-1)of DDP groups and the cells were treated for 6,12,24,and 48 h.The suitable concentration of DDP(6 μmol·L^-1) and culture time(24 h) were chosen.The Hep-2 cells treated under the suitable condition were randomly divided into control group,DDP group,resveratrol group,sirtinol group,resveratrol+sirtinol group,resveratrol+DDP group,sirtinol+DDP group and resveratrol+sirtinol+DDP group. The survival rates of the Hep-2 cells in various groups were detected by CCK-8 assay;the expression levels of Sirt1 protein in the Hep-2 cells in various groups were determined with immunofluorescence method;the apoptotic rates of the Hep-2 cells in various groups were detected by Annexin Ⅴ-FITC Kit.Results The CCK-8 assay results showed that the survival rates of the Hep-2 cells in resveratrol group and DDP group were decreased significantly compared with control group(P<0.01);compared with DDP group,the survival rates of the Hep-2 cells in resveratrol+DDP group were decreased(P<0.01);compared with resveratrol+DDP+sirtinol group,the survival rates of the Hep-2 cells in resveratrol+DDP group were decreased(P<0.05).The immunofluorescence results showed that the mean fluorescence intensity of Sirt1 protein of the cells in resveratrol+DDP group were increased significantly compared with DDP group(P<0.01);compared with resveratrol+sirtinol+DDP group,the mean fluorescence intensity of Sirt1 protein of the cells in resveratrol+DDP group was increased significantly (P<0.01).The Annexin Ⅴ-FITC Kit results showed the apoptotic rate of the cells in resveratrol+DDP group was higher than those in DDP group and resveratrol+sirtinol+DDP group(P<0.01).Conclusion Resveratrol can improve the sensitivity of Hep-2 cells to cisplatin,and its mechanism may be related to increasing the expression of Sirt1 protein.

Key words: resveratrol, silent information regulator 1, laryngeal neoplasms, Hep-2 cells, cisplatin, sirtinol

CLC Number:

R739.65

ZHAN Shihua, WANG Ping, YIN Wanzhong, ZHU Wei. Enhancement effect of resveratrol on sensitivity of laryngeal carcinoma Hep-2 cells to cisplatin and its mechanism[J].Journal of Jilin University Medicine Edition, 2015, 41(02): 282-286.

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Enhancement effect of resveratrol on sensitivity of laryngeal carcinoma Hep-2 cells to cisplatin and its mechanism

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