p-Tau蛋白在抑郁症大鼠海马组织中的表达

doi:10.13481/j.1671-587x.20150208

Abstract

Abstract:

Objective To observe the expression level of the phosphorylated microtubule-associated protein Tau (p-Tau) in the hippocampus tissue of the rats with depression,and to explore the relationship between p-Tau and depression.Methods 40 rats were randomly divided into control group (n=20) and model group (n=20).The depression models of rats were established by chronic unpredictable mild stress (CUMS),and the rats in control group were caught once a day.The behavior examination was carried out in control group and model group by sucrose preference test,open field test,and Morris water maze.The morphology of hippocampal neurons of the rats was observed by Nissl staining.At the 1st,7th,14th,and 28th day after CUMS,the rats in control group and model group were executed in batches and the expression levels of p-Tau protein in the hippocampus tissue of the rats were detected by Western blotting method.Results The sucrose preference test results showed that the sucrose consumption and the percentage of the sucrose preference of the rats in model group were lower than those in control group (P<0.01);the open field test results showed the overall walking distance,the central activity time,the up-right times,and the grooming behavior of the rats in model group were lower than those in control group (P<0.01);the Morris water maze results showed the average escape latent period of the rats in model group was higher than that in control group (P<0.01).The expression level of p-Tau protein in the hippocampus tissue of the rats in model group was lower than that in control group 1 d after CUMS(P<0.01),and was higher than that in control group after 7 d(P<0.05);the expression level of p-Tau protein in the hippocampus tissue of the rats in model group was the highest 14 d after CUMS(P<0.01).Conclusion The expression level of p-Tau protein in the hippocampus tissue of the rats with depression is decreased firstly and then increased,which may affect the stability of microtubule and destroy the steady state of cytoskeleton,thus leading to the structural changes of the hippocampal neurons.

Key words: depression, hippocampus, microtubule-associated protein, rats,SD

CLC Number:

R749

SUI Zhuxin, LI Zhen, LIU Hao, YUAN Yang, WANG Haitao. Expression of p-Tau protein in hippocampus tissue of rats with depression[J].Journal of Jilin University Medicine Edition, 2015, 41(02): 245-248.

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Expression of p-Tau protein in hippocampus tissue of rats with depression

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