严重烧伤对大鼠海马神经细胞自噬和神经胶质细胞反应的诱导作用

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Induction of severe burn on autophagy of nerve cells and astrocyte reaction in hippocampus tissue of rats

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doi:10.13481/j.1671-587x.20150503

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Objective To observe the changes of nerve cells in hippocampus tissue of severe burn rats,and to discuss its possible mechanism. Methods 20 adult Wistar rats were randomly divided into normal control group (n=10) and model group (n=10).The rats in model group were used to establish the severe burn models.1 week after modeling,the eating and drinking state of rats in two groups were observed.The pathohistology of nerve cells in hippocampus tissue of rats were observed by HE and Nissl staining;the expressions of glial fibrillary acidic protein (GFAP),microtubule-associated protein-2 (MAP-2),light chain 3B (LC3B) and Beclin 1 were detected by immunohistochemistry method. Results The rats in normal control group ate and drank regularly,but the rats in model group were listless and inactive after burning.HE and Nissl staining results showed that the disappearance and disorder of Nissl bodies were observed in model group,and the nerve cells in hippocampus tissue of severe burn rats partly damaged with cellular swelling and nuclear pyknosis.There was no obvious damage of the nerve cells in normal control group.The immunohistochemistry results showed that the expression levels of GFAP,LC3B,and Beclin 1 in the rats in model group were significantly higher than those in normal control group (P<0.05),and the expression level of MAP-2 was significantly lower than that in normal control group (P<0.05). Conclusion After severe burn,the hippocampus nerve cells of rats are damaged,which may result in astrocyte reaction and the increasing of nerve cells autophagy.

Key words: burn, hippocampus, nerve cells damage, astrocyte reaction, autophagy

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R995

LI Lei, WANG Yongjie, CHEN Hao, QIN Yonggang, ZHOU Yubo, ZHAO Jiaxun, GUO Li. Induction of severe burn on autophagy of nerve cells and astrocyte reaction in hippocampus tissue of rats[J].Journal of Jilin University Medicine Edition, 2015, 41(05): 898-901.

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