Abstract:

Objective To observe the effects of exogenous expression of mutamer of vascular endothelial growth factor VEGF165b on the damage of human bladder cancer T24 cells induced by cisplatin(DDP),and to discuss its mechanism. Methods The VEGF165b expression vector pcDNA-VEGF165b was constructed.According to the processing method,T24 cells were divided into control group,VEGF165b group (transfected with VEGF165b expression vector),DDP group (treated with DDP),DDP+VEGF165b group (DDP plus transfected with VEGF165b expression vector).MTT method was used to detected the cell viability;Western blotting method was used to detect the expression of apoptotic proteins Bcl-2,Bax,and caspase3;TUNEL method was used to detect the number of apoptotic cells. Results The MTT results showed that compared with control group,the cell viability in VEGF165b group at 24 and 48 h had no significant changes;compared with DDP group, the cell viability at 24 and 48 h in VEGF165b+DDP group were significantly decreased (P<0.05).The Western blotting results showed that compared with control group,the value of cleaved-caspase3/pro-caspase3 in T24 cells in DDP group was increased (P<0.05),and the value of Bcl-2/Bax was decreased (P<0.05);compared with DDP group,the value of cleaved-caspase3/pro-caspase3 in cells in VEGF165b+DDP group was increased (P<0.05),and the value of Bcl-2/Bax was decreased (P<0.05).The TUNEL results showed that compared with control group (5.1±2.7) and VEGF165b group (7.4±3.2),the number of apoptotic cells in DDP group (26.3±8.2) was increased (P<0.05).Compared with DDP group,the number of apoptotic cells in VEGF165b+DDP group (41.3+6.9) was increased (P<0.05). Conclusion The expression of exogenous VEGF165b can promote the damage of DDP on T24 cells by increasing the apoptosis of T24 cells,and its mechanism is related to the inhibition of VEGF siginal passway.

Key words: bladder neoplasms, vascular endothelial growth factor, cisplatin