siRNA干预树突状细胞CD40表达对大鼠炎症性肠病的治疗作用

doi:10.13481/j.1671-587x.20150111

Abstract

Abstract:

Objective To use small interfering RNA (siRNA) to silence the expression of CD40 molecule in dendritic cells (DCs), and to observe its effect on inflammatory bowel disease (IBD) in the rats. Methods A highly efficient siRNA carrier and eukaryotic expression vector pcDNA3-CD40 were designed and synthesized, and they were transfected into DCs of the rats respectively to build the inflammatory bowel disease (IBD) models in the rats.18 Wistar rats were divided into control group, intervention group and overexpression group .All rats models were built using trinitro-benzene -sulfonic acid(TNBS).After successful construction, the rats in control group were injected intraperitoneally with normal saline, the rats in intervention group were injected intraperitoneally with interference expression vector, and the rats in overexpression group were injected intraperitoneally with pcDNA3-CD40.The clinical symptoms such as diarrhea and mucopurulent bloody stool were observed after 15 d. Results The interference expression vector was constructed successfully.The expression levels of CD40 mRNA and protein in DCs were both reduced detected by PCR and Western blotting method.The transfected DCs were successfully acted on model rats.The clinical symptoms such as diarrhea, mucus purulent blood in intervention group were improved significantlycompared with control group.The symptoms such as diarrhea and mucopurulent bloody stool were deteriorated in overexpression group, of which two rats were dead. Conclusion The intervened expression of CD40 in DCs with siRNA has a therapeutic effect on IBD in rats.

Key words: inflammatory bowel disease, dendritic cells, small interfering RNA, CD40

CLC Number:

R-332

WANG Liying, YANG Yong, LI Zhandong, LI Dongfu, LIU Libin, WANG Min. Therapentic effect of siRNA inhibiting CD40 expression in dendritic cells on inflammatory bowel disease in rats[J].Journal of Jilin University Medicine Edition, 2015, 41(01): 54-59.

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Therapentic effect of siRNA inhibiting CD40 expression in dendritic cells on inflammatory bowel disease in rats

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