Journal of Jilin University Medicine Edition ›› 2015, Vol. 41 ›› Issue (01): 60-63.doi: 10.13481/j.1671-587x.20150112

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Protective effects of 17-β estradiol on apoptosis of retinal ganglion cells induced by pressure and its mechanism

WANG Ying1, XU Wenjing1, GAO Zhizhuo2, HU Ping1, YANG Haiyan1, RAO Wei1, XU Shaolin1   

  1. 1. Department of Ophthalmology, Sencond Hospital, Jinlin University, Changchun 130041, China;
    2. Department of Ophthalmology, Genenral Hospital of CNPC, Jilin 132013, China
  • Received:2014-06-16 Published:2015-01-30

Abstract:

Objective To explore the protective effects of 17-β estradiol(E2) on the apoptosis of cultured retinal ganglion cells(RGC-5 cells) induced by pressure, and to clarify its mechanism. Methods The RGC-5 cells were divided into control group, 80 mmHg pressure group and 80 mmHg pressure combined with E2 group.The cells at logarithmic growth phase were used in the experiment.The cell viabilities in various groups were determined by MTT assay.The apoptotic morphology of the cells in various groups was determined by Hoe33342 staining.The expression levels of bax, bcl-2, P53, iASPP, and cleaved-caspase-3were determined by Western blotting method. Results The MTT assay showed that compared with control group, the viabilities of the RGC-5 cells in pressure group and pressure combined with E2 group were decreased(P<0.05); compared with pressure group, the viability of the RGC-5 cells in pressure combined with E2 was increased(P<0.05).The results of cell staining showed that compared with pressure group, the chromatin condensation and formation of apoptotic bodies in the RGC-5 cells in pressure combined with E2 group were decreased.The Western blotting results showed that compared with control group, the expression levels of bax, P53 and cleaved-caspase-3 were increased(P<0.05), the expression levels of bcl-2 and iASPP were decreased(P<0.05) in the RGC-5 cells in pressure group and pressure combined with E2 group;compared with pressure group, the expression levels of bax, P53 and cleaved-caspase-3 were decreased(P<0.05), and the expression levels of bcl-2 and iASPP were increased(P<0.05) in the RGC-5 cells in pressure combined with E2 group. Conclusion E2 has the protective effects on the RGC-5 cells and could decrease the apoptosis of RGC-5 cells under pressure loading condition.

Key words: 17-&beta, estradiol, retinal ganglion cells, pressure, apoptosis

CLC Number: 

  • R774.6