神经干细胞移植联合促红细胞生成素对大鼠脊髓损伤的修复作用

doi:10.13481/j.1671-587x.20150225

Abstract

Abstract:

Objective To observe the repair effects of neural stem cells(NSCs) combined with erythropoietin (EPO) on transverse spinal cord injury in the adult rats,and to provide a theoretical basis for clinical treatment of spinal cord injury.Methods Forty Wistar rats were used to set up T10 spinal cord transaction models and were randomly divided into control group(n=10),NSCs group(n=10),EPO group(n=10), and NSCs+EPO group(n=10).NF-200 immunohistochemistry and immunofluorescence staining were used to detect the morphology of the regenerating nerve fibers of the rats in various groups 8 weeks after the operation;the BBB score was used to evaluate the restoration of motor function of the hind limbs of the rats in various groups.Results The results of histomorphology showed that the stump of the transected spinal cord tissue atrophied,and there were a large amount of the formation of hole between white matter and gray matter of spinal cord of the rats in control group.The regeneration of nerve fibers could not be seen clearly of the rats in control group.The stump of transected spinal cord tissue atrophied slightly of the rats in NSCs+EPO group,and a large amount of nerve fibers with messy and disorderly state were visible in the cross-sectional area,and there was a lot of continuous nerve fibers getting through the cross-sectional area,and a small number of the formation of hole between white matter and gray matter of spinal cord could be observed.In the experiment,a lot of FITC-conjugated anti-neurofilament antibody NF-200 labelled nerve fibers regenerated in the side of head of the transected area in NSCs+EPO group could be observed,which grew disorderly,and got through the tail side of the damage zone;However,there was a small number of the regeneration of nerve fibers in NSCs group,and these fibers didn't get through the damage zone.The BBB scores of motor function of the hind limbs of the rats in NSCs+EPO group were higher than those in other groups within 7 d after the operation(P<0.05),and the BBB scores of the rats in NSCs group were also higher than those in control group and EPO group(P<0.05).Conclusion NSCs transplantation combined with intraperitoneal injection of EPO could promote the recovery of the neurological function and benefit the survival and regeneration of injured axons after spinal cord injury in the adult rats.

Key words: neural stem cells, erythopoietin, cell transplantation, spinal cord injury

CLC Number:

R651.2

ZHAO Yan, XIAO Yulong, ZUO Yuan, WANG Xiliang, HUO Hongjun, JIANG Jianming, YAN Huibo. Repair effect of neural stem cells transplantation combined with erythropoietin on spinal cord injury in rats[J].Journal of Jilin University Medicine Edition, 2015, 41(02): 333-337.

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Repair effect of neural stem cells transplantation combined with erythropoietin on spinal cord injury in rats

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