Journal of Jilin University Medicine Edition ›› 2015, Vol. 41 ›› Issue (02): 327-332.doi: 10.13481/j.1671-587x.20150224

Previous Articles     Next Articles

Therapeutic effect of arterial infusion chemotherapy on glioma of rats and its mechanism

WANG Kunpeng1,2, ZHAO Wenqing2,3, WANG Weixing1, HU Tiemin1, KANG Jinsheng3, SUN Xin4, JU Zhiqing5   

  1. 1. Department of Neurosurgery, Affiliated Hospital, Chengde Medical University, Chengde 067000, China;
    2. Graduate School, Hebei Medical University, Shijiazhuang 050017, China;
    3. Department of Neurosurgery, Hebei General Hospital, Shijiazhuang 050051, China;
    4. Department of Neurosurgery, WeiChang Manchu-Mongolian Autonomous County Hospital, Chengde 068450, China;
    5. Department of Rehabilitation, Affiliated Hospital, Chengde Medical University, Chengde 067000, China
  • Received:2014-09-03 Online:2015-03-28 Published:2015-04-04

Abstract:

Objective To study the therapeutic effect and mechanism of arterial infusion chemotherapy on glioma of the rats,and to provide the basis for clinical application.Methods 24 rats were randomly divided into artery group,vein group,and control group; there were 8 rats in each group.The chemotherapy medicine VM-26 were injected through tail intravenous(vein group) and carotid arteries (artery group) into the rats, respectively,and the rats in control group were given normal saline. The tumor size and survival period of the rats were observed;all the rats were killed after giving different treatment for one week, and the expression levels of proliferating cell nuclear antigen(PCNA) and topoisomeraseⅡ(TOPO Ⅱ) in the tumor cells of the rats were detected by PCR method,meanwhile the number of PCNA positive cells in the tumor cells were detected by immunohistochemical method;the apoptotic rates of the tumor cells of the rats were detected by flow cytometry method.Results After treatment,the tumor growth speed of the rats in artery group was the slowest,which was followed by vein group, and the speed of the rats in control group was the fastest.The tumor size of the rats in artery group was smaller than those in vein and control groups,and the tumor size of the rats in vein group was smaller than that in control group(P<0.05).Compared with control group,the survival period of the rats in artery and vein groups were extended (P<0.05);the survival period of the rats in artery group was longer than that in vein group,but there was no statistically significant difference between two groups (P>0.05).The expression levels of PCNA and TOPO Ⅱ mRNA in the tumor tissue of the rats in artery group were lower than those in vein and control groups (P<0.05),and the expression levels of PCNA and TOPO Ⅱ in the tumor tissue of the rats in vein group were lower than those in control group (P<0.05).The number of PCNA positive cells in the tumor tissue of the the rats in artery group was lower than those in vein group and control group (P<0.05).The apoptotic rate of tumor cells of the rats in artery group was higher than those in vein group and control group(P<0.05).Conclusion The way of arterial perfusion chemotherapy could make the proliferation of tumor cells slow,and promote the apoptosis of tumor cells obviously,and can control the tumor growth more effectively.

Key words: glioma, arterial infusion, proliferating cell nuclear antigen, topoisomerase Ⅱ, apoptosis, chemotherapy

CLC Number: 

  • R361.3