组蛋白去乙酰化酶抑制剂SAHA对卵巢癌SKOV3细胞的化疗增敏作用

doi:10.13481/j.1671-587x.20180303

Abstract

Abstract: Objective: To investigate the biological effects of histone deacetylase inhibitors(HDACIs) suberoylanilide hydroxamic acid(SAHA) combined with vincristine (VCR) on the ovarian cancer SKOV3 cells, and to clarify their mechanisms. Methods: The human ovarian cancer SKOV3 cells were divided into control group,SAHA group,VCR group and SAHA+VCR group.CCK-8 method was used to detect the surival rates of SKOV3 cells in various groups.The apoptotic rates,the autophagy rates and the distribution of cell cycles of SKVO3 cells in various groups were detected by flow cytometry (FCM). Results: The CCK-8 results showed that compared with control group,the survival rates of SKOV3 cells in SAHA group and VCR group were decreased significantly.Compared with VCR group or SAHA group,the survival rates of cells in SAHA+VCR groups were decreasedsignificantly(P<0.05).The FCM results demonstrated that compared with control group, the apoptotic rates,the autophagy rates and the percentages of the SKOV3 cells at G₂/M phase in SAHA group and VCR group were increased(P<0.05);compared with SAHA or VCR groups, the apoptotic rate,the autophagy rate and the percentages of SKOV3 cells at G₂/M phase in SAHA+VCR groups were increased(P<0.05). Conclusion: SAHA can enhance the sensitivity of ovarian cancer SKOV3 cells to VCR and induce the apoptosis and autophagy of SKOV3 cells to promote the cell arrest at G₂/M phase.The combined application of SAHA and chemotherapeutic drugs may be a new strategy for the treatment of ovarian cancer patients.

Key words: vincnistine, suberoylanilide hydroxamic acid, ovarian neoplasms, sensitivity

CLC Number:

R737.31

LIANG Bing, LIU Yang, SONG Jinping, QU Danhua, LIU Chunyan. Enhancement of histone deacetylase inhibitors SAHA in chemotherapeutic sensitivity of ovarian cancer SKOV3 cells[J].Journal of Jilin University Medicine Edition, 2018, 44(03): 471-476.

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Enhancement of histone deacetylase inhibitors SAHA in chemotherapeutic sensitivity of ovarian cancer SKOV3 cells

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