萱草花总黄酮含药血清对肝癌HepG2细胞增殖和凋亡的影响

doi:10.13481/j.1671-587x.20160420

Abstract

Abstract:

Objective: To explore the effects of serum containing hemerocallis citrine baroni flavonids (HCBF) on the proliferation and apoptosis of human hepatoma HepG2 cells,and to clarify their mechanisms. Methods: The HepG2 cells were divided into blank control group,low (900 mg·kg^-1),middle (1 800 mg·kg^-1) and high (2 700 mg·kg^-1) doses of serum containing HCBF groups. The inhibitory rates of growth of HepG2 cells were measured with MTT assay; inverted microscope was used to observe the morphologic changes; Annexin Ⅴ-PI double staining and flow cytometry were used to detect apoptotic rates of HepG2 cells;enzyme linked immunosorbent assay was used to detect the expression levels of Bax,bcl-2, and Caspase-3. Results: Compared with blank control group,the inhibitory rates of growth of HepG2 cells in middle and high doses of HCBF groups were increased (P < 0.05 or P < 0.01).The cells were shrank with irregular shape,and the nucleus was condensed and broken in HCBF group under inverted microscope.Compared with blank control group,the apoptotic rates of HepG2 cells in middle and high doses of HCBF groups were increased (P < 0.05 or P < 0.01). Compared with blank control group,the expression levels of bcl-2 protein in middle and high doses of HCBF groups were decreased(P < 0.05 or P < 0.01),and the expression levels of Bax protein were significantly increased (P < 0.05 or P< 0.01),and the expression level of Caspase-3 protein in high dose of HCBF group was significantly increased(P < 0.05). Conclusion: The serum containing HCBF could induce the apoptosis of HepG2 cells,and its mechanism may be related to mitochondrial pathway.

Key words: hemerocallis citrine baroni flavonids, serum pharmacology, HepG2 cells, cell proliferation, apoptosis

CLC Number:

R7285.5

SHEN Nan, CHEN Qian, QI Ling, CHEN Xue, HUANG Chengran, TANG Hairu, AN Ying, LIN Feng, WAN Yanchun. Effects of serum containing hemerocallis citrine baroni flavonids on proliferation and apoptosis of hepatoma HepG2 cells[J].Journal of Jilin University Medicine Edition, 2016, 42(04): 737-741.

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Effects of serum containing hemerocallis citrine baroni flavonids on proliferation and apoptosis of hepatoma HepG2 cells

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