浆细胞样树突状细胞和Foxp3+调节性T细胞在结直肠癌组织中的表达及其意义

doi:10.13481/j.1671-587x.20200427

Abstract

Abstract: Objective: To investigate the expressions of CD123⁺ plasmacytoid dendritic cells(pDCs) and Foxp3⁺ regulatory cells(Tregs) in the colorectal cancer tissue (CRC) and their infiltrations in the tumor draining lymph nodes (TDLN), and to elucidate their effects on the biological behaviors of CRC. Methods: A total of 63 pathological specimens of removed CRC tissue, cancer adjacent normal mucosa tissue and TDLN were collected. The number of CD123⁺pDCs and Foxp3⁺Treg positive cells in different tissues were detected by immunohistochemical method. The correlation between the expressions of them were detected by Spearman analysis. Results: The number of Foxp3⁺Tregs positive cells in cancer tissue of the CRC patients was higher than that in adjacent normal mucosa tissue(P<0.01). The number of Foxp3⁺Tregs positive cells in cancer tissue of the patients with positive lymph node metastasis was higher than that in adjacent normal mucosa (P<0.01).The number of Foxp3⁺Tregs positive cells in metastatic TDLN (mTDLN) was higher than that in metastatic free TDLN (mfTDLN) (P<0.01).The number of Foxp3⁺Tregs psitive cells in cancer tissue in the patients in TNM Ⅲ+Ⅳstage was higher than that in the patients in TNM Ⅰ+Ⅱ stage (P<0.01).The number of CD123⁺pDCs positive cells in cancer tissue of the CRC patients was lower, and the positive expression rate of CD123 in CRC tissue was signifificantly higher than that in adjacent normal mucosa tissue (P<0.01).The number of CD123⁺pDCs positive cells in mfTDLN and mTDLN had no significant difference (P>0.05).The pDCs/myeloid dendritic cells(mDCs) ratio in mTDLN was signifificantly higher than that in mfTDLN(P<0.01).The correlative analysis results demonstrated that the ratio of pDCs/mDCs had a positive relationship with the number of Foxp3⁺ Tregs positive cells(r=0.421, P<0.01). Conclusion: The occurrence and development of CRC has nothing to do with the expression frequency of CD123⁺pDCs and is related to the amount of Foxp3⁺Tregs infiltration in the cancer tissue,The change of DCs subgroups in tumor tissue has a positive relationship with Foxp3⁺Tregs, which promotes the occurrence and development of CRC.

Key words: plasmacytoid dendritic cells, tumor microenvironment, regulatory T cells, colorectal neoplasms

CLC Number:

R735.3

WANG Dan, LIAO Dan, LI Hong, XIONG Liqiu, WU Ying, DONG Ying, GAI Xiaodong. Expressions of plasmacytoid dendritic cells and Foxp3⁺ regulatory T cells in colorectal cancer tissue and their significances[J].Journal of Jilin University(Medicine Edition), 2020, 46(04): 834-838.

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Expressions of plasmacytoid dendritic cells and Foxp3⁺ regulatory T cells in colorectal cancer tissue and their significances

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Expressions of plasmacytoid dendritic cells and Foxp3+ regulatory T cells in colorectal cancer tissue and their significances

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Expressions of plasmacytoid dendritic cells and Foxp3⁺ regulatory T cells in colorectal cancer tissue and their significances