关键词: 生物工程, 组织培养, 组合设计, 愈伤组织, 培养基

Abstract: Experimental materials are cotyledon of American ginseng/s(Panax quin quefolium linne) seed embryo, hypocotyl of carrot/s（Daucus carota）seedling fro m seed. Experimental design is the orthogonal design combination with quadratic regression. The experiment has 17 treatments with 3 factors those were as follow s: 2,4D, IAA and ZT. Every factor supposed 5 levels. First, the regression pa rameters of the growth rate were carried out by SPSS statistical analysis softwa re based on test results. Then, tendency diagrams of the growth rates for two pl ants callus were drawn up by the Matlab software. Finally，the media with differ ent function were chosen. The callus of the American ginseng preferred the mediu m（MS+2,4D1.14 mg/L +IAA1.37 mg/L+ZT0.47 mg/L）.For the carrot, the greater ra te of the callus growth was seen in the medium (MS+2,4D 3.30 mg/L+IAA 0.55 mg/L+ZT 0.29 mg/L). The rate of induction and growth of callus for both American ginseng and carrot was higher in the medium (MS+ 2,4D 1.67 mg/L+IAA 0.98 mg/L+ZT 0.47 mg/L).

Key words: bioengineering, tissue culture, design combination, callus, medium