人参β-AS基因RNAi表达载体工程菌的构建

摘要/Abstract

摘要： 利用重组PCR和酶切连接技术构建了人参β-香树素合成酶(β-AS)基因RNAi(RNA interference, RNAi)植物表达载体,再经热转化法转化到发根农杆菌A4菌株中,得到含有人参β-AS基因RNAi植物表达载体的工程菌,为诱导转化人参外植体奠定了基础。

关键词: 生物工程, 人参皂苷, β-香树素合成酶, RNAi植物表达载体, 工程菌

Abstract: In this study, the ginseng β-AS gene RNA interference (RNAi) plant expression vector is built through the Recombinant PCR reaction and restriction enzyme digestion connection. Then,by heating, the β-AS gene RNAi is transformed into the Agrobacterium A4 to get β-AS RNAi plant expression vector engineered bacteria. This lays the foundation for further induced transformation of ginseng explants.

Key words: bioengineering, ginsenoside, β-AS, RNAi plant expression vector, engineering bacteria

中图分类号:

赵寿经, 曹豪杰, 何沐阳, 王乐. 人参β-AS基因RNAi表达载体工程菌的构建[J]. , 2012, (06): 1608-1612.

ZHAO Shou-jing, CAO Hao-jie, HE Mu-yang, WANG Le. Engineering bacteria construction of ginseng β-AS gene RNA interference plant expression vector[J]. , 2012, (06): 1608-1612.

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