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基于简单统计排名模型的差异表达基因识别

吴佳楠1,2, 周春光1,3, 夏雪飞4, 刘桂霞1,3, 沈薇1, 周柚1,3   

  1. 1. 吉林大学 计算机科学与技术学院,长春 130012;
    2. 长春大学 计算机科学与技术学院,长春 130022;
    3. 吉林大学 符号计算与知识工程教育部重点实验室,长春 130012;
    4. 吉林交通职业技术学院,长春 130012
  • 收稿日期:2011-09-20 出版日期:2013-07-01 发布日期:2013-07-01
  • 通讯作者: 刘桂霞(1963-),女,教授.研究方向:计算智能和生物信息学.E-mail:liugx@jlu.edu.cn E-mail:liugx@jlu.edu.cn
  • 作者简介:吴佳楠(1980-),男,博士研究生,讲师.研究方向:计算智能和生物信息学.E-mail:jiananwu@126.com
  • 基金资助:

    国家自然科学基金项目(60873146, 60803052, 60973092, 60903097);吉林省科技发展计划青年研究项目(201201139, 20090116, 20101589);吉林省教育厅"十二五"科学技术研究项目(256).

Using simple statistical model to identify differentially expressed genes in microarray experiments

WU Jia-nan1,2, ZHOU Chun-guang1,3, XIA Xue-fei4, LIU Gui-xia1,3, SHEN Wei1, ZHOU You1,3   

  1. 1. College of Computer Science and Technology, Jilin University, Changchun 130022, China;
    2. College of Computer Science and Technology, Changchun University, Changchun 130022, China;
    3. Symbolic Computation and Knowledge Engineering Laboratory of the Ministry of Education, Jilin University, Changchun 130022, China;
    4. Jilin Communications Polytechnic College, Changchun 130012, China
  • Received:2011-09-20 Online:2013-07-01 Published:2013-07-01

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摘要：

不同实验条件下差异表达基因(DEGs)的识别是微阵列数据分析的主要目标之一,针对分析结果中具有高排名的基因往往表现出较低差异表达水平的缺点,提出了一种基于简单统计排名模型的差异表达基因识别算法MRP(Matrix rank product)。算法可直接处理基因芯片原始数据,排除了数据预处理方法对算法的干扰;另外,通过对基因芯片数据形成的矩阵进行整体排序计算,得到具有高准确度的差异表达性排名结果。

关键词: 计算机应用, 生物信息学, 差异表达基因识别, 基因芯片数据, 排名

Abstract:

One of the main objectives in the analysis of microarray data is the identification of Differentially Expressed Genes (DEGs) under different experiment conditions. A main approach for such analysis is to calculate a statistical value for each gene, and then rank the genes in accordance with their statistical values. A large ranking value is evidence of a differential expression. Inevitably, different methods generally produce different gene rankings, and the performance of each method depends on its evaluation metric, the dataset and data preprocessing method. A disadvantage shared by existing methods is that some top ranked genes, which are falsely detected as DEGs, tend to exhibit lower expression levels. Here, we present a novel technique named Matrix Rank Product (MRP) for identifying differentially expressed genes that originate from a simple statistical rank model. The algorithm can directly deal with the raw data of the microarray. As a result it can eliminate the interference of different data preprocessing methods. Meanwhile, the new technique is designed for accurate gene ranking by calculating the microarray data matrix of overall sorting.

Key words: computer application, bioinformatics, identification of differentially expressed genes, microarray data, rank

中图分类号: 

  • TP399

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