吉林大学学报(医学版) ›› 2021, Vol. 47 ›› Issue (3): 623-629.doi: 10.13481/j.1671-587X.20210311

• 基础研究 • 上一篇    下一篇

黄芩苷联合二甲双胍对多囊卵巢综合征大鼠内分泌、炎症因子和胰岛素抵抗的影响及其机制

谢磊,刘晓丽,葛静,李亚威()   

  1. 河北医科大学第一医院妇科,河北 石家庄 050031
  • 收稿日期:2020-10-23 出版日期:2021-05-28 发布日期:2021-05-28
  • 通讯作者: 李亚威 E-mail:nihao123338@sina.com
  • 作者简介:谢 磊(1979-),女,河北省石家庄市人,主治医师,医学硕士,主要从事妇科疾病基础和临床方面的研究。
  • 基金资助:
    河北省卫健委医学科学研究项目(20190419)

Effects of baicalin combined with metformin on endocrine, inflammatory factors and insulin resistance in rats with polycystic ovary syndrome and their mechanisms

Lei XIE,Xiaoli LIU,Jing GE,Yawei LI()   

  1. Department of Gynecology,First Hospital,Hebei Medical University,Shijiazhuang 050031,China
  • Received:2020-10-23 Online:2021-05-28 Published:2021-05-28
  • Contact: Yawei LI E-mail:nihao123338@sina.com

摘要: 目的

探讨黄芩苷(BAL)联合二甲双胍(DMBG)对多囊卵巢综合征(PCOS)大鼠内分泌指标和炎症因子表达、子宫内膜情况和胰岛素抵抗的影响及其机制。

方法

将32只大鼠随机分为对照组(给予生理盐水)、模型组[(给予脱氢表雄酮(DHEA)]、二甲双胍组(给予DMBG)和联合组(给予BAL+DMBG),每组8只。除正常组外,其他各组大鼠采用DHEA诱导建立PCOS大鼠模型。大鼠腹主动脉取血,采用ELISA法检测各组大鼠血清黄体生成素(LH)、卵泡刺激素(FSH)、睾酮(T)、雌二醇(E2)和肿瘤坏死因子α(TNF-α)、C反应蛋白(CRP)、白细胞介素6(IL-6)及白细胞介素18(IL-18)水平,采用放射免疫法检测各组大鼠空腹胰岛素(FINS)和空腹血糖(FBG)水平,并计算胰岛素抵抗指数(HOMA-IR),检测各组大鼠子宫内膜隙/间质面积比和子宫内膜厚度,Western blotting法检测各组大鼠卵巢组织中磷酸化磷脂酰肌醇-3-激酶(p-PI3K)、磷脂酰肌醇-3-激酶(PI3K)、磷酸化蛋白激酶B(p-AKT)和蛋白激酶B(AKT)蛋白表达量。

结果

与对照组比较,DHEA组大鼠血清FSH和E2水平降低(P<0.05),LH、T、TNF-α、CRP、IL-6和IL-18水平升高(P<0.05),血清中FBG、FINS水平和HOMA-IR明显升高(P<0.05),子宫内膜隙/间质面积比和子宫内膜厚度明显减少(P<0.05);与DHEA组比较,BAL+DMBG组和DMBG组大鼠血清FSH、E2、TNF-α、CRP、IL-6及 IL-18水平升高(P<0.05),血清LH、T、FBG和FINS水平及HOMA-IR降低(P<0.05),子宫内膜隙/间质面积比和子宫内膜厚度增加(P<0.05);与DMBG组比较,BAL+DMBG组大鼠血清FSH、E2、TNF-α、CRP、IL-6和 IL-18水平升高(P<0.05),血清LH、T、FBG和FINS水平及HOMA-IR降低(P<0.05),子宫内膜隙/间质面积比和子宫内膜厚度增加(P<0.05)。

结论

BAL联合DMBG可通过上调PCOS大鼠卵巢组织中PI3K/AKT信号通路蛋白表达水平,纠正内分泌紊乱,抑制慢性低度炎症反应,改善胰岛素抵抗及子宫内膜情况。

关键词: 黄芩苷, 二甲双胍, 多囊卵巢综合征, 炎症因子, 胰岛素抵抗, 子宫内膜

Abstract: Objective

To investigate the effects of baicalin (BAL)combined with metformin(DMBG) on the endocrine indicatros, inflammatory factors, endometrial condition and insulin resistance of the rats with polycystic ovary syndrome (PCOS) and their mechanisms.

Methods

A total of 32 rats were divided into control group, dehydroepiandrosterone(DHEA) group, DMBG group, and BAL + DMBG group,and there were 8 rats in each group.Except control group,the rats in other groups were given DHEA to establish the PCOS models.The blood of abdominal aorta of the rats was taken, and the levels of serum endocrine factorsluteinizing hormone(LH), follicle-stimulating hormone(FSH), testosterone(T),estradiol(E2)and tumor necrosis factor-α(TNF-α),C-reactive protein(CRP), interleukin 6(IL-6)and interleukin 18(IL-18)were determined by enzyme-linked immunosorbent assay (ELISA)method; radioimmunoassay was used to detect the levels of fasting insulin(FINS)and fasting blood-glucose(FBG),and the insulin resistance index(HOMA-IR) was calculated;the area ratio of endometrial space to stroma and thickness of endometrium of the rats in various groups were detected;Western blotting method was used to detect the expression amounts of phosphorylated phosphatidylinositide 3-kinases (p-PI3K),phosphatidylinositide 3-kinases(PI3K), phosphorylated protein kinase B (p-AKT) and protein kinase B (AKT) in ovary tissue of the rats in various groups.

Results

Compared with control group, the serum levels of FSH and E2 of the rats in DHEA group were decreased(P<0.05),and the serum levels of LH,T, TNF-α, CRP, IL-6,and IL-18 were increased (P<0.05),the serum levels of LH,T,FBG,FINS and HOMA-IR were increased (P<0.05),and the area ratio of endometrial space to stroma and the thickness of endometrium were decreased(P<0.05).Compared with DHEA group, the serum levels of FSH, E2, TNF-α, CRP, IL-6,and IL-18 of the rats in BAL+DMBG group and DMBG group were increased (P<0.05),the serum levels of LH,T,FBG,FINS, and HOMA-IR were decreased (P<0.05),and the area ratios of endometrial space to stroma and the thicknesses of endometrium were increased(P<0.05).Compared with DMBG group, the serum levels of FSH, E2, TNF-α, CRP, IL-6,and IL-18 of the rats in BAL+DMBG were increased (P<0.05),the serum levels of LH,T,FBG,FINS, and HOMA-IR were decreased (P<0.05), and the area ratio of endometrial space to stroma and the thickness of endometrium were increased(P<0.05).

Conclusion

BAL combined with DMBG can improve insulin resistance and endometrium condition by up-regulating the expressions of PI3K / AKT signaling pathway proteins in ovarian tissue of the PCOS rats,correcting endocrine disorders,and inhibiting the chronic low-grade inflammatory reaction.

Key words: baicalin, metformin, polycystic ovary syndrome, inflammatory factor, insulin resistance, endometrium condition

中图分类号: 

  • R711.76