The C2C12 cells were divided into control group and different concentrations （50，75，100，and 125 μmol·L^－1） of PA groups when the C2C12 cells grew to 70%－80%. The C2C12 cells in different concentrations of PA groups were given growth media containing different concentrations of PA， while the C2C12 cells in control group were not treated with PA（0 μmol·L^－1 PA）.CCK-8 method was used to detect the cell proliferation activities of cells in various groups，the triglyceride （TG） levels in the C2C12 cells in various groups were detected， Oil Red O staining was used to observe the formation of lipid droplets in the C2C12 cells in various groups， and 2% horse serum was used to induce the cell differentiation and evaluate the differentiation of C2C12 cells in various groups.

Compared with control group， there were no significant differences in the proliferation activities of the C2C12 cells in 50， 75， and 100 μmol·L^－1 PA groups（P>0.05）， the proliferation activity of the C2C12 cells in 125 μmol·L^－1 PA group was decreased（P<0.01），the TG levels in the C2C12 cells in 50， 75 and 100 μmol·L^－1 PA groups were increased （P<0.01）， and the TG level in the C2C12 cells in 100 μmol·L^－1 PA group was increased significantly （P<0.01）.Compared with cultured for 24 h， the TG level in the C2C12 cells after treated with 100 μmol·L^－1 PA for 48 h was increased （P<0.01）. After differentiation and culture， more myotubes were formed in the C2C12 cells in PA groups and control group.

The lipid deposition in the C2C12 cells could be effectively promoted after cultured with 100 μmol·L^－1 PA for 48 h.