关键词: 瘦素, 乳腺肿瘤, 细胞凋亡, 信号通路

Abstract:

Objective To observe the effects of leptin on the proliferation and apoptosis of the breast carcinoma MCF-7 cells, and to explore the mechanism. Methods The MCF-7 cells at logarithm growth phase were selected and randomly divided into control group and different doses (20, 40, 80 μg·L^-1) of leptin groups.The proliferation rates of the MCF-7 cells in various groups were determined by CCK8 assay kit;the apoptotic rates of the MCF-7 cells in various groups were analyzed by flow cytometry;the mRNA and protein expressions of bcl-2 and bax of the MCF-7 cells in various groups were detected by RT-PCR and Western blotting methods;the protein expressions of p-AKT and bcl-2 in the MCF-7 cells in different cell signaling pathway inhibitor treatment groups were tested by Western blotting method. Results Compared with control group, the proliferation rates of the MCF-7 cells in leptin groups were increased in a dose-dependent manner(P<0.05);the apoptotic rates of the MCF-7 cells in leptin groups were decreased(P<0.05);the mRNA and protein expression levels of bax in the MCF-7 cells in leptin groups were decreased(P<0.05), and the mRNA and protein expression levels of bcl-2 were increased(P<0.05);compared with leptin group, the protein expressions of p-AKT and bcl-2 in PI3K-AKT inhibitor LY294002 group were markedly decreased(P<0.05). Conclusion Leptin can increase the proliferation and inhibit the apoptosis of breast carcinoma MCF-7 cells, and the mechanism of anti-apoptosis may be releted to the up-regulation of bcl-2 via PI3K-AKT signaling pathway.

Key words: leptin, breast carcinoma, apopotosis, signaling pathway