抑制TRIM24基因表达对乳腺癌MCF-7细胞增殖、凋亡、侵袭和迁移的抑制作用

doi:10.13481/j.1671-587x.20190631

吉林大学学报(医学版) ›› 2019, Vol. 45 ›› Issue (06): 1379-1383.doi: 10.13481/j.1671-587x.20190631

抑制TRIM24基因表达对乳腺癌MCF-7细胞增殖、凋亡、侵袭和迁移的抑制作用

杨庄青, 陆眉, 杨晓娟, 王常安, 邹洁雅

昆明医科大学第三附属医院云南省肿瘤医院乳腺二科, 云南昆明 650118

收稿日期:2018-12-27 出版日期:2019-12-05 发布日期:2019-12-05
作者简介:杨庄青(1974-),女,苗族,湖南省凤凰县人,副教授,医学硕士,主要从事乳腺肿瘤和乳房整形方面的研究。
基金资助:
云南省自然科学基金资助课题（2017FZ239）

Inhibitory effect of inhibiting TRIM24 gene expression on proliferation, apopotosis,invasion and migration of breast cancer MCF-7 cells

YANG Zhuangqing, LU Mei, YANG Xiaojuan, WANG Chang'an, ZOU Jieya

Department of Breast Surgery, Third Affiliated Hospital, Kunming Medical University, Yunnan Cancer Hospital, Kunming 650118, China

Received:2018-12-27 Online:2019-12-05 Published:2019-12-05

摘要/Abstract

摘要： 目的：探讨抑制TRIM24基因表达对乳腺癌MCF-7细胞增殖、凋亡、侵袭和迁移的影响，阐明TRIM24基因在乳腺癌发生发展中的作用。方法：以脂质体法转染TRIM24 siRNA和阴性对照NC-siRNA至MCF-7细胞作为si-TRIM24组和NC-siRNA组，以未转染的MCF-7细胞作为对照组。qRT-PCR和Western blotting法检测转染48 h后各组MCF-7细胞中TRIM24 mRNA和蛋白表达水平，MTT法检测各组MCF-7细胞增殖能力，流式细胞术检测各组MCF-7细胞凋亡率，Transwell实验检测各组MCF-7细胞侵袭和迁移数。结果：与对照组和NC-siRNA组比较，si-TRIM24组MCF-7细胞中TRIM24 mRNA和蛋白表达水平明显降低（P<0.05），MCF-7细胞增殖能力降低（P<0.05），MCF-7细胞凋亡率明显升高（P<0.05），MCF-7细胞侵袭和迁移数明显减少（P<0.05）。结论：抑制TRIM24基因表达能够明显抑制乳腺癌MCF-7细胞的增殖、侵袭和迁移，且能够诱导细胞凋亡。

关键词: TRIM24基因, 乳腺肿瘤, 细胞增殖, 细胞凋亡, 侵袭, 迁移

Abstract: Objective: To investigate the effect of inhibiting TRIM24 gene expression on the proliferation, apoptosis, invasion and migration of breast cancer MCF-7 cells, and to elucidate the role of TRIM24 gene in the occurrence and development of breast cancer. Methods: The TRIM24 siRNA and negative control NC-siRNA were transfected into the MCF-7 cells by liposome method, and used as si-TRIM24 group and NC-siRNA group, respectively. The untransfected MCF-7 cells were used as control group. qRT-PCR and Western blotting methods were used to detect the expression levels of TRIM24 mRNA and protein in the MCF-7 cells in various groups after transfected for 48 h,MTT assay was performed to detect the proliferation activities of the MCF-7 cells in various groups,flow cytometry was used to determine the apoptotic rates of the MCF-7 cells in various groups,and Transwell assay was used to measure the number of invasion and migration cells in various groups. Results: Compared with control group and NC-siRNA group, the expression levels of and protein in the MCF-7 cells were significantly decreased (P<0.05), and the proliferation activity of the MCF-7 cells was decreased (P<0.05), the apoptotic rate of the MCF-7 cells was significantly increased (P<0.05), and the number of invasion and migration MCF-7 cells was significantly decreased (P<0.05). Conclusion: Inhibition of the expression of TRIM24 gene can significantly inhibit the proliferation, invasion and migration of breast cancer MCF-7 cells, and induce the apoptosis.

Key words: TRIM24 gene, breast neoplasms, cell proliferation, apoptosis, invasion, migration

中图分类号:

R737.9

杨庄青, 陆眉, 杨晓娟, 王常安, 邹洁雅. 抑制TRIM24基因表达对乳腺癌MCF-7细胞增殖、凋亡、侵袭和迁移的抑制作用[J]. 吉林大学学报(医学版), 2019, 45(06): 1379-1383.

YANG Zhuangqing, LU Mei, YANG Xiaojuan, WANG Chang'an, ZOU Jieya. Inhibitory effect of inhibiting TRIM24 gene expression on proliferation, apopotosis,invasion and migration of breast cancer MCF-7 cells[J]. Journal of Jilin University(Medicine Edition), 2019, 45(06): 1379-1383.

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抑制TRIM24基因表达对乳腺癌MCF-7细胞增殖、凋亡、侵袭和迁移的抑制作用

Inhibitory effect of inhibiting TRIM24 gene expression on proliferation, apopotosis,invasion and migration of breast cancer MCF-7 cells

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