miR-367对人乳腺癌MCF-7细胞增殖、侵袭和迁移的影响及其机制

doi:10.13481/j.1671-587x.20190627

吉林大学学报(医学版) ›› 2019, Vol. 45 ›› Issue (06): 1353-1360.doi: 10.13481/j.1671-587x.20190627

miR-367对人乳腺癌MCF-7细胞增殖、侵袭和迁移的影响及其机制

周春¹, 王光华², 张帮柱²

1. 攀枝花学院临床医学院外科教研室, 四川攀枝花 617000;
2. 攀枝花学院附属医院普外科, 四川攀枝花 617000

收稿日期:2018-11-30 出版日期:2019-12-05 发布日期:2019-12-05
通讯作者: 周春,讲师(Tel:0812-3370556,E-mail:zhouchun112@126.com) E-mail:zhouchun112@126.com
作者简介:周春(1974-),女,四川省射洪市人,讲师,医学硕士,主要从事普外科疾病基础和临床方面的研究。
基金资助:
四川省教育厅科研项目资助课题（16ZB0480）；四川省攀枝花市科技局科技计划项目资助课题（2015CY-S-33）

Effects of miR-367 on proliferation, invasion and migration of human breast cancer MCF-7 cells and their mechanisms

ZHOU Chun¹, WANG Guanghua², ZHANG Bangzhu²

1. Department of Surgery, School of Clinical Medine, Panzhihua University, Panzhihua 617000, China;
2. Department of General Surgery, Affiliated Hospital, Panzhihua University, Panzhihua 617000, China

Received:2018-11-30 Online:2019-12-05 Published:2019-12-05

摘要/Abstract

摘要： 目的：探讨miR-367对人乳腺癌MCF-7细胞增殖、侵袭和迁移的影响，并阐明其作用机制。方法：检测乳腺癌组织、癌旁组织、MCF-7细胞和MCF-10A细胞中miR-367相对表达水平。将miR-NC或miR-367-inhibitor转入MCF-7细胞作为阴性对照组和miR-367-inhibitor组，检测2组MCF-7细胞活性、细胞集落数、Ki67阳性表达率、细胞划痕愈合率、侵袭细胞数和KLF4相对表达水平。将miR-NC或miR-367-mimic转入MCF-7细胞作为阴性对照组和miR-367-mimic组，检测2组MCF-7细胞集落数、细胞划痕愈合率、侵袭细胞数和KLF4相对表达水平。结果：与癌旁组织或MCF-10A细胞比较，乳腺肿瘤组织和MCF-7细胞中miR-367相对表达水平均明显升高（P<0.01）。与阴性对照组比较，miR-367-inhibitor组MCF-7细胞中miR-367相对表达水平、细胞活性、细胞集落数、Ki67阳性表达率、细胞划痕愈合率和侵袭细胞数均明显降低（P<0.01），KLF4相对表达水平明显升高（P<0.01）；与阳性对照组比较，miR-367-mimic组MCF-7细胞中miR-367相对表达水平、细胞集落数、细胞划痕愈合率和侵袭细胞数均明显升高（P<0.01），KLF4相对表达水平明显降低（P<0.01）。结论：miR-367可促进MCF-7细胞增殖、侵袭和迁移，其机制可能与抑制KLF4的表达有关。

关键词: 微小RNA-367, Krüppel样因子4, 乳腺肿瘤, 细胞增殖, 迁移, 侵袭

Abstract: Objective: To investigate the effects of miR-367 on the proliferation, invasion and migration of the human breast cancer MCF-7 cells, and to elucidate their mechanisms. Methods: The relative expression levels of miR-367 in the breast cancer tissues, adjacent tissues, MCF-7 cells and MCF-10A cells were detected. The MCF-7 cells were transfected with miR-NC or miR-367-inhibitor as negative control group and miR-367-inhibitor group, and the cell viabilities, the number of colonies, the positive expression rates of Ki67, the rate of cell wound healing, the number of invasive cells and the relaitive expression levels of KLF4 in the MCF-7 cells in two groups were detected. The MCF-7 cells were transfected with miR-NC or miR-367-mimic as negative control group and miR-367-mimic group, and the number of colonies, the rates of cell wound healing, the number of invasive cells and the relaitive expression levels of KLF4 in the MCF-7 cells in two groups were detected. Results: Compared with adjacent tissue or MCF-10A cells, the relative expression levels of miR-367 in breast cancer tissues and MCF-7 cells were significantly increased (P<0.01).Compared with negative control group,the relative expression level of miR-367 in the MCF-7 cells, the cell viability,the number of colonies,the positive expression rate of Ki67, the rate of cell wound healing and the number of invasive cells in miR-367-inhibitor group were significantly decreased (P<0.01), and the relative expression level of KLF4 was significantly increased (P<0.01);compared with positive control group,the relative expression level of miR-367 in the MCF-7 cells, the number of colonies, rates of cell wound healing and number of invasive cells in miR-367-mimic group were significantly increased (P<0.01), and the relative expression level of KLF4 was significantly decreased (P<0.01). Conclusion: miR-367 promotes the proliferation, migration and invasion of MCF-7 cells, and the mechanism may be related to the inhibition of KLF4 expression.

Key words: microRNA-367, Kruppel factor 4, breast neoplasms, cell proliferation, migration, invasion

中图分类号:

R737.9

周春, 王光华, 张帮柱. miR-367对人乳腺癌MCF-7细胞增殖、侵袭和迁移的影响及其机制[J]. 吉林大学学报(医学版), 2019, 45(06): 1353-1360.

ZHOU Chun, WANG Guanghua, ZHANG Bangzhu. Effects of miR-367 on proliferation, invasion and migration of human breast cancer MCF-7 cells and their mechanisms[J]. Journal of Jilin University(Medicine Edition), 2019, 45(06): 1353-1360.

参考文献

[1] DESANTIS C, MA J, BRYAN L, et al. Breast cancer statistics, 2013[J].CA Cancer J Clin, 2014, 64(1): 52-62.
[2] BRAY F, FERLAY J, SOERJOMATARAM I, et al. Global cancer statistics2018:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries [J]. CA Cancer J Clin, 2018, 68(6): 394-424.
[3] FAN L, STRASSER-WEIPPL K, LI J J, et al. Breast cancer in China [J]. Lancet Oncol, 2014, 15(7): e279-e289.
[4] DESANTIS C E, MA JM, GODING SAUER A, et al. Breast cancer statistics, 2017, racial disparity in mortality by state [J]. CA Cancer J Clin, 2017, 67(6): 439-448.
[5] ZUO T T, ZHENG R S, ZENG H M, et al. Female breast cancer incidence and mortality in China, 2013[J]. Thorac Cancer, 2017, 8(3): 214-218.
[6] OUZOUNOVA M, VUONG T, ANCEY P B, et al. MicroRNA miR-30 family regulates non-attachment growth of breast cancer cells [J]. BMC Genomics, 2013, 14(1): 139.
[7] 陈万青, 郑荣寿. 中国女性乳腺癌发病死亡和生存状况[J]. 中国肿瘤临床, 2015, 42(13): 668-674.
[8] REDDY K B. MicroRNA (miRNA) in cancer [J]. Cancer Cell Int, 2015, 15(1): 38.
[9] RUPAIMOOLE R, CALIN G A, LOPEZ-BERESTEIN G, et al. MiRNA deregulation in cancer cells and the tumor microenvironment [J]. Cancer Discov, 2016, 6(3): 235-246.
[10] TUTAR L, ÖZGÜ R A, TUTAR Y. Involvement of miRNAs and pseudogenes in cancer [J]. Methods Mol Biol, 2018, 1699: 45-66.
[11] XU J, WU W B, WANG J, et al. MiR-367 promotes the proliferation and invasion of non-small cell lung cancer via targeting FBXW7[J]. Oncol Rep, 2017, 37(2): 1052-1058.
[12] DING D X, ZHANG Y, WEN L, et al. MiR-367 regulates cell proliferation and metastasis by targeting metastasis-associated protein 3(MTA3) in clear-cell renal cell carcinoma [J]. Oncotarget, 2017, 8(38): 63084-63095.
[13] MENG X, LU P, FAN Q. miR-367 promotes proliferation and invasion of hepatocellular carcinoma cells by negatively regulating PTEN [J]. Biochem Biophys Res Commun, 2016, 470(1): 187-191.
[14] ZHANG B,HE D D,FANG X J, et al. The microRNA-367 inhibits the invasion and metastasis of gastric cancer by directly repressing Rab23[J]. Genet Test Mol Biomarkers, 2015,19(2): 69-74.
[15] JIA Y L, ZHOU J C, LUO X, et al. KLF4 overcomes tamoxifen resistance by suppressing MAPK signaling pathway and predicts good prognosis in breast cancer [J]. Cell Signal, 2018, 42: 165-175.
[16] SONG X, XING Y M, WU W, et al. Expression of Krüppel-like factor 4 in breast cancer tissues and its effects on the proliferation of breast cancer MDA-MB-231 cells [J]. Exp Ther Med, 2017, 13(5): 2463-2467.
[17] WANG G C, HE Q Y, TONG D K, et al. MiR-367 negatively regulates apoptosis induced by adriamycin in osteosarcoma cells by targeting KLF4[J]. J Bone Oncol, 2016, 5(2): 51-56.

miR-367对人乳腺癌MCF-7细胞增殖、侵袭和迁移的影响及其机制

Effects of miR-367 on proliferation, invasion and migration of human breast cancer MCF-7 cells and their mechanisms

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