吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (04): 725-730.doi: 10.13481/j.1671-587x.20160418

• 基础研究 • 上一篇    下一篇

补骨脂乙素对舌鳞状细胞癌Tca8113细胞增殖的抑制作用和凋亡诱导作用及其机制

史毅1, 金晓红2, 吴伟忠1, 霍安1, 周伟1, 黄汉3   

  1. 1. 江苏省丹阳市人民医院口腔科, 江苏 丹阳 212300;
    2. 江苏省丹阳市云阳人民医院内科, 江苏 丹阳 212300;
    3. 辽宁医学院附属第一医院口腔科, 辽宁 锦州 121001
  • 收稿日期:2015-08-30 发布日期:2016-07-20
  • 通讯作者: 黄汉,副主任医师,硕士研究生导师(Tel:0416-4197262,E-mail:125391956@qq.com) E-mail:125391956@qq.com
  • 作者简介:史毅(1987-),男,江苏省丹阳市人,医师,医学硕士,主要从事口腔颌面部恶性肿瘤方面的研究。
  • 基金资助:

    辽宁省教育厅普通高校学科带头人基金资助课题(kk0023)

Effects of isobavachalcone on proliferation and apoptosis of tongue squamous cell carcinoma Tca-8113 cells and their mechanisms

SHI Yi1, JIN Xiaohong2, WU Weizhong1, HUO An1, ZHOU Wei1, HUANG Han3   

  1. 1. Department of Stomatology, Danyang People's Hospital, Jiangsu Province, Danyang 212300, China;
    2. Department of Internal Medicine, Yunyang People's Hospital of Danyang City, Jiangsu Province, Danyang 212300, China;
    3. Department of Stomatology, First Affiliated Hospital, Liaoning Medical College, Jinzhou 121001, China
  • Received:2015-08-30 Published:2016-07-20

摘要:

目的:观察补骨脂乙素(IBC)对人舌鳞状细胞癌Tca8113细胞增殖和凋亡的影响,并阐明其可能的作用机制。方法:将体外培养的Tca8113细胞分为对照组(0 μmol·L-1)和不同浓度IBC组(10、20、40及80 μmol·L-1)。通过MTT法检测细胞增殖抑制率,流式细胞术检测细胞凋亡率,Western blotting法检测各组细胞中Akt、p-Akt、Erk、p-Erk、Bax、Bcl-2和Caspase-3蛋白的表达水平。结果:MTT法检测,随着IBC浓度的增加和作用时间的延长,Tca8113细胞的增殖抑制率逐渐增加,12、24和48h半数抑制浓度(IC50)分别为(285.13±8.97)、(132.40±7.76)和(58.56±5.93) μmol·L-1,不同时间点IC50比较差异有统计学意义(P < 0.05)。流式细胞术检测,与对照组(1.69%±0.65%)比较,作用48h后20和40 μmol·L-1 IBC组细胞凋亡率(分别为8.21%±2.32%和22.45%±1.18%)明显升高(P < 0.05)。Western blotting法检测,与对照组比较,作用48h后20和40 μmol·L-1 IBC组细胞中Bcl-2、p-Akt和p-Erk表达水平明显降低(P < 0.05),Bax表达水平明显升高(P < 0.05);Akt和Erk蛋白表达水平无明显变化(P > 0.05);与对照组比较,作用48h后40 μmol·L-1 IBC组Tca8113细胞中Caspase-3蛋白表达水平明显升高(P < 0.05)。结论:IBC可抑制Tca8113细胞的增殖并诱导细胞凋亡,Akt和Erk通路可能是其诱导肿瘤细胞凋亡的途径。

关键词: 补骨脂乙素, 舌肿瘤, 癌, 鳞状细胞, 细胞增殖, 细胞凋亡

Abstract:

Objective: To observe the effects of isobavachalcone (IBC) on the proliferation and apoptosis of tongue squamous cell carcinoma Tca-8113 cells,and to explore their mechanisms. Methods: The Tca8113 cells cultured in vitro were divided into control group and different doses (0,10,20,40,80 μmol·L-1) of IBC groups. The inhibitory rates of cell proliferation were detected by MTT method.The apoptosis was detected by flow cytometry.Western blotting method was used to detect the expressions of Akt,p-Akt,Erk,p-Erk,Bax,Bcl-2 and Caspase-3 proteins in the Tca8113 cells in various groups. Results: The MTT results showed that the inhibitory rates of proliferation of Tca8113 cells were increased in a concentration- and time- dependent manner;the IC50 at 12,24 and 48 h were (285.13±8.97),(132.40±7.76), and (58.56±5.93) μmol·L-1, respectively;and there were significant differences between different time points (P < 0.05).The FCM results showed that the apoptotic rates of Tca8113 cells in 20 and 40 μmol·L-1 IBC groups at 48 h were (8.21±2.32)% and (22.45±1.18)%, respectively;compared with control group (1.69%±0.65%),the differences were statistically significant (P < 0.05). The Western blotting results showed that the expression levels of Bcl-2,p-Akt, and p-Erk, in 20 and 40 μmol·L-1 IBC groups were decreased and the expression levels of Bax were increased compared with control group (P < 0.05);the expression levels of Akt and Erk had no significant changes(P > 0.05).The expression levels of Caspase-3 in Tca8113 cells in 40 μmol·L-1 IBC group at 48 h were increased compared with control group (P < 0.05). Conclusion: IBC could inhibit the proliferation and induce the apoptosis of Tca8113 cells;Akt and Erk signaling pathway may be the pathway of IBC to induce the apoptosis of tumor cells.

Key words: isobavachalcone, tongue neoplasms, carcinoma,squamous cell, cell proliferation, apoptosis

中图分类号: 

  • R739.86